Identification of Streptomyces sp. AMLK-335 Producing Antibiotic Substance Inhibitory to Vancomycin-Resistant Enterococci

  • Published : 2001.06.01

Abstract

The actinomycete strain AMLK-335 was antagonistic to vancomycin-resistant enterococci (VRE). Based on the diaminopimelic acid (DAP) type, and morphological and physiological characteristics revealed by scanning electron microscopy (SEM), AMLK-335 was confirmed to belong to the genus Streptomyces. Analysis of the 16S rDNA nucleotide sequences found AMLK-335 to have a relationship with Streptomyces platensis. The production of antibiotic from this strain was most favorable when cultured on glucose, polypeptone, yeast extract (PY) medium for 6 days at $27^{\circ}$. The antibiotic was identified as cyclo(L-phenylalanyl-L-prolyl) by comparing ti with the reported MS and NMR spectral data. Cyclo(phe-pro) from the PY cultures of AMLK-335 was most effective (K-98-258). Futhermore, cyclo(phe-pro) had antimicrobial activity against Bacillus subtilis, Microcuccs luteus, Staphylococcus aureus, and Saccharomyces cerevisiae, but it wa ineffective against Candida albicans, Streptomyces murinus, and Aspergillus niger.

Keywords

References

  1. Mortal. Weekly Rep. v.45 Nosocomial enterococci resistant to vancomycin-United States Centers for Disease Control
  2. Bull. Agric. Chem. Soc. Jpn. v.24 Studies on the metabolic products of Roselinia necatrix. . Isolation and characterization of several physiologically active neutral substances Chen, Y.-S.
  3. Drugs v.51 no.SUP.1 Emerging resistance to antimicrobial agents in gram-positive bacteria: Enterococci, staphylococci and nonpneumococcal streptococci Cormican, M. G.;R. N. Jones
  4. Appl. Microbiol. v.21 Classification of Streptomyces spore surfaces into five groups Dietz, A.;J. Mathew
  5. Clin. Infect. Dis. v.16 Nosocomial outbreak due to Enterococcus faecium highly resistant to vancomycin, penicillin, and gentamicin Handwerger, S.;B. Raucher;D. Altarac;J. Monka;S. Marchione;K. Y. Singh;B. E. Murray;J. Wolff;B. Walters
  6. Trends Biochem. Sci. v.23 no.10 Multiple sequence alignment with Clustal X. Jeanmougin, F.;G. Orsini
  7. Diagn. Microbiol. Infect. Dis. v.21 Emerging multiple resistant enterococci among clinical isolates. Ⅰ. Prevalence data from 97 medical center surveillance study in the United States Jones, R. N.;H. S. Sader;M. E. Erwin;P. Nygron
  8. J. Microbiol. Biotechnol. v.10 Comparison of the solution structure of vancomycin with its X-ray crystallographic structure Lee, C.-H.;H.-S. Kyung;Y.-H. Lim
  9. Kor. J. Appl. Microbiol. v.24 Isolation and identification of Streptoverticillium sp. NA-4803 producing antifungal substance Lim, D.-S.;S.-K. Yoon;M.-S. Lee;W.-H. Yoon;C.-H. Kim
  10. Infect. Control Hosp. Epidemiol. v.18 Hospital - acquired infection with vancomycin resistant Enterococcus faecium transmitted by electronic ear prove thermometer Livornese, L. L.;S. Dias;C. Samuel
  11. Med. J. Aust. v.164 Emerging resistance in Enterococcus sp. Martin, C.;M. G. Cormican;Ronald, N. Jones
  12. Clin. Infect. Dis. v.14 Emergence of Enterococcus as a significant pathogen Moellering, Jr. R. C.
  13. Ann. Intern. Med. v.123 Enterococci resistant to multiple antimicrobial agents, including vancomycin. Establishment of endemicity in a university medical center Morris, J. G.;D. K. Shay;J. N. Hebden;M. Arther
  14. Clin. Microbiol. Rev. v.3 The life and times of the enterococcus Murray, B. E.
  15. Peptides v.16 no.1 Bioactive cyclic dipeptide Prasad, C.
  16. Appl. Microbiol. v.6 A guide for classification of streptomycetes according to selected groups placement of strains in morphological sections Pridham, T. G.;C. W. Hesseltine;R. G. Benedict
  17. Mol. Biol. Evol. v.4 The neighbor joining method: A new method for constructing phylogenetic tree Saitou, N.;M. Nei
  18. Int. J. Syst. Bacteriol. v.16 Methods for characterization of Streptomyces sp. Shirling, E. B.;D. Gottlieb
  19. Ann. Intern. Med. v.125 A comparison of the effect of universal glove and gown use with glove use alone in the acquisition of vancomycin-resistant enterococci in a medical intensive care unit Slaughter, S.;N. K. Hayden;C. Nathan
  20. J. Microbiol. Biotechnol. v.9 Characterization of the replication region of the Enterococcus faecalis plasmid p703/5 Song, J.-S.;J.-H. Park;C.-H. Kim;Y.-W. Kim;W.-J. Lim;I.-Y. Kim;H.-I. Chang
  21. Proc. Natl. Acad. Sci. v.91 Hospital acquired infections: Disease with increasingly limited therapies Swartz, M. N.
  22. JAMA v.274 The prevalence of noscomical infection in intensive care units in Europe Vincent, J. L.;D. J. Bihari;P. M. Suter
  23. Bergey's Manual of Systematic Bacteriology v.4 Williams, S. T.;M. E. Sharpe;J. G. Holt;R. G. E. Murray;D. J. Brener;N. R. Krieg;J. W. Mouldar;N. Pfennig;P. H. A. Sneath;J. T. Staley
  24. J. Gen. Microbiol. v.48 Use of a scanning electron microscope for the examination of Actinomycetes Williams, S. T.;F. L. Davies
  25. Clin Microbiol. Rev. v.8 Current perspectives on glycopeptide resistance Woodford, N.;A. P. Johnson
  26. J. Gen. Appl. Microbiol. v.16 Taxonomic studies on Coryneform bacteria. Ⅱ. Principle amino acids in the cell wall and their taxonomic significance Yamada, K.;K. Kamagata