Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

White Spot Syndrome Virus in Penaeid Shrimp Cultured in Korea

  • Shin, En-Joo (Department of Biology, Seoul Women's University) ;
  • Park, Jae-Hak (Department of Laboratory Animal Science, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Yeon-Hee (Department of Biology, Seoul Women's University)
  • Published : 2001.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Because of the great concern over the possibility of contamination from the rod-shaped nuclear virus (PRDV) from Japan and white spot virus (WSSV) from Taiwan, most eggs used in Korean shrimp farms are currently obtained from local broodstock. In addition, the screening of imported broodstock for any viral presence at the National Fisheries Research and Development Institute is also mandatory. Nonetheless, massive mortality from white spot syndrome continues in Korea. In the present study, we present an improved PCR method to use tissue-extracted DNA instead of viral DNA extracted from a purified virus based on a sucrose density gradient, and produced results within 8 h. In 1998, this modified PCR method was able to detect that diseased Penaeus japonicus were infected within 8 h. In 1998, this modified PCR method was able to detect that diseased Penaeus japonicus were infected only with PRDV, while Fenneropenaeus chinensis were infected with both PRDV and WSSV. In 1999, PRDV and WSSV were detected in F. chinensis with signs of infection, but not with WSSV alone.

Keywords

References

  1. Asian Shrimp News v.21 Mass mortality caused by systemic baciliform virus in cultured penaeid shrimp, Penaeus monodon, in Thailand Asian Shrimp Culture Council
  2. J. Virol. Methods v.34 Methods for viral isolation and DNA extraction for a penaeid shrimp baculovirus Bruce, L. D.;B. B. Trumper;D. V. Lightner
  3. J. Fish China v.19 Epidemiological studies on the explosive epidemic disease of prawn in 1993-1994 Cai, S.;J. Huang;C. Wang;X. Song;X. Sun;J. Yu;Y. Zhang;C. Yang
  4. Aquaculture v.164 Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crab and lobsters in in situ hybridization Chang, P. C.;H. C. Chen;Y. C. Wang
  5. Dis. Aquat. Org. v.23 Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan Chou, H.Y.;C. Y. Huang;C. H. Wang;H. C. Chiang;C. F. Lo
  6. Aquaculture v.164 Studies on transmission of white spot syndrome associated baculovirus (WSSV) in Penaeus monodon and P. japonicus via waterborne contact and oral ingestion Chou, H. Y.;C. Y. Huang;C. F. Lo;G. H. Kou
  7. Marine Fish Res. v.16 Baculoviral hypodermal and hematopoietic necrosis - study on the pathogen and pathology of the explosive epidemic disease of shrimp Huang, J.;X. Song;J. Yu;C. Yang
  8. Fish Pathol. v.29 Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: Electron microscopic evidence of the causative virus Inouye, K.;S. Miwa;N. Oseko;H. Nakano;T. Kimura T.
  9. Fish Pathol. v.31 The penaeid rod-shaped DNA virus (PRDV), which causes penaeid acute viremia (PAV) Inouye, K.;K. Yamano;N. Ikeda;T. Kimura;H. Nakano;K. Momoyama;J. Kobayashi;S. Mihajima
  10. Aquaculture v.164 Detection of white-spot syndrome in cultured penaeid shrimp in Asia: Microscopic observation and polymerase chain reaction Kasornchandra, J.;S. Boonyaratpalin;T. Itami
  11. J. Fish Diseases v.21 Development of a polymerase chain reaction (PCR) procedure for the detection of baculovirus associated with white spot syndrome (WSSV) in penaeid shrimp Kim, C. K.;P. K. Kim;S. G. Sohn;D. S. Sim;M. A. Park;M. S. Heo;T. H. Lee;J. D. Lee;H. K. Jun;K. L. Jang
  12. J. Microbiol. Biotechnol. v.10 In situ hybridization of white spot disease virus in experimentaly infected Penaeid shrimp Lee, W. W.;B. J. Lee;Y. Lee;Y. S. Lee;J. H. Park
  13. A Handbook of Shrimp Pathology and Diagnostic Procedures for Disease of Cultured Penaeid Shrimp Lightner, D. V.
  14. Dis. Aquat. Org. v.25 Detection of baculovirus associated with white spot syndrome (WSSV) in penaeid shrimp using polymerase chain reaction Lo, C. F.;J. H. Leu;C. H. Ho;C. H. Chen;S. E. Peng;Y. T. Chen;C. M. Chou;P. Y. Yeh;C. J. Huang;H. Y. Chou;C. H. Wang;G. H. Kou
  15. Dis. Aquat. Org. v.30 Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs Lo, C. F.;C. H. Ho;C. H. Chen;K. F. Liu;Y. I. Chiu;P. Y. Yeh;S. E. Peng;H. C. Hsu;H. C. Liu;C. F. Chang;M. S. Su;C. H. Wang;G. H. Kou
  16. Fish Pathol. v.29 Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: histopathological study Momoyama, K.;M. Hiraoka;H. Nakano;H. Koube;K. Inouye;N. Oseka
  17. Fish Pathol. v.29 Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: epizootiological survey and infection trials Nakano, H.;H. Kouble;S. Umezaea;K. Momoyama;M. Hiraoka;K. Inouye;N. Oseko
  18. Dis. Aquat. Org. v.34 An infectious virus disease of penaeid shrimp newly found in Korea Park, J.;S. Lee;Y. Lee
  19. Fish Pathol. v.292 Electron microscopic evidence of bacilliform virus infection in Kuruma shrimp (Penaeus japonicus) Takahashi, Y.;T. Itami;M. Kondo;m. Maeda;R. Fujii;S. tomonaga;K. Supamattaya;S. Boonyaratpalin
  20. J. Fish Diseases v.19 Polymerase chain reaction (PCR) amplification of bacilliform virus (PRDV) DNA in Penaeus japonicus Bate and systemic ectodermal and mesodermal baculovirus (SEMBV) DNA in Penaeus monodon Fabricus Takahashi, Y.;T. Itami;M. Maeda;N. Suzuki;J. Kasornchandra;K. Supamattaya;R. Khongpradit;S. Boonyaratpalin;M. Kondo;K. Kawai;R. Kusuda;I. Hirono;T. Aoki
  21. J. Virol. Methods v.82 A polymerase chain reaction protocol for the detection of various geographical isolates of white spot virus Tapay, L. M.;E. C. Nadala;P. C. Loh
  22. Dis. Aquat. Org. v.23 Purification and genornic analysis of baculovirus associated with white spot syndrome (WSSV) of Penaeus monodon Wang, C. H.;C. F. Lo;J. H. Leu;C. M. Chou;P. Y. Yeh;H. Y. Chou;M. C. Tung;C. F. Chang;M. S. Su;G. H. Kou
  23. Dis. Aquat. Org. v.21 A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal orgin and causes high mortality in the black tiger prawn Penaeus monodon Wongteerasupaya, C.;J. E. Vicker;S. Sriurairatana;G. L. Nash;A. Akarajamorn;V. Boonsaeng;S. Panyim;A. Tassanakajon;B. Withyachumnarnkul;T. W. Flegel TW.