Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression Analysis of phbC Coding for Poly-3-hydroxybutyrate (PHB) Synthase of Rhodobacter sphaeroides

  • Published : 2001.04.01
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Abstract

Poly-3-hydroxybutyrate (PHB) synthase catalyzed the last enzymic step to synthesize the intracellular PHB of Rhodobacter sphaeroides. No PHB was detected when the phbC coding for PhB synthase was interrupted, and its expression was regulated at the level of transcription. The cellular PHB content increased about four- to six-fold during the growth transition from the exponential to the early stationary phase under both aerobic and photoheterotrophic conditions. The PHB content during the aerobic growth seemed to be determined by the PhB synthase activity. However, the PHB synthase activity of photoheterotrophically grown cells did not correlate with the PhB content, suggesting a photoheterotrophic regulation different from the aerobic control. Thus, the PHB content of R. sphaeroides was regulated at the transcription level only under aerobic conditions.

Keywords

References

  1. Micorbiol. Rev. v.54 Occurrence metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates Anderson, J. A.;E. A. Dawes
  2. J. Micorbiol. Biotechnol. v.9 Production of poly(3-hydroxybutyrate) [P(3HB)] with high P(3HB) content by recombinant Escherichia coli hardboring the Alcaligenes latus P(3HB) biosynthesis genes and the E. coli ftsZ gene Choi, J. I.;S. Y. Lee
  3. J. Microbiol. Biotechnol. v.9 Isolation of a Pseduomonas sp. strain exhibiting unusual behavior of poly(3-hydroxybutyrates) biosynthesis and characterizatin of synthesized polyesters Chung, C. W.;Y. S. Kim;Y. B. Kim;K. S. Bae;Y. H. Rhee
  4. J. Bacteriol. v.170 In vivo analysis of puf operon expression in Rhodobacter sphaeroides after deletion of a putative intercistronic transcription terminator DeHoff, B. S.;J. K. Lee;T. J. Donohue;R. I. Gumport;S. Kaplan
  5. J. Bacteriol. v.170 Construction, characterization, and complementation of a puf mutant of Rhodobacter sphaeroides Davis, T.;T. J. Donohue;S. Kaplan
  6. J. Bacteriol. v.168 Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides cytochrome c₂ gene Donohue, T. J.;A. G. McEwan;S. Kaplan
  7. Biochemistry v.27 Phenotypic and genetic characterization of cytochrome c₂-deficient mutant of Rhodobacter sphaeroides Donohue, T. J.;A. G. McEwan;S. Kaplan
  8. Marcromol. Chem. Rapid Commum. v.8 Biosynthesis of terpolyesters of 3-hydroxybutyrate, and 5-hydroxyvarate in Acaligenes eutrophus from 5-chloropentanoic and pentanoic acids Doi, Y.;A. Tamaki;M. Kunioka;K. Sopa
  9. J. Bacteriol. v.176 prrA, a putative response regulator involved in oxygen regulation of photosynthesis gene expression in Rhodobacter sphaeroides Eraso, J. M.;S. Kaplan
  10. Proc. Natl. Acad. Sci. USA v.76 Replication of an origin containing derivative of plasmid RK2 dependent on a plasmid function provided in trans Figurski, D. H.;D. R. Helinski
  11. FEMS Microbiol. Lett. v.93 Cloning of poly(3-hydroxybutyric acid) synthase genes of Rhodobacter sphaeroides and Rhodospirillum rubrum and heterologous expression in Alcaligenes eutrophus Hustede, E.;A. Steinbuchel;H. G. Schlegel
  12. Biotechnol. Lett. v.15 Characterization of the polyhydroxyalkanoate synthase gene locus of Rhodobacter sphaeroides Hustede, E.;A. Steinbuchel
  13. Appl. Microbol. Biotechnol. v.39 Relationship between the photoproduction of hydrogen and the accumulation of PHB in non-sulphur purple bacteria Hustede, E.;A. Steinbuchel;H. G. Schlegel
  14. Gene v.70 Improved broad-host range plasmids for DNA cloning in gram-negative bacteria Keen, N. T.;S. Tamaki;D. Kobayashi;D. Trollinger
  15. J. Bacteriol. v.169 Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides light-harvesting B-800-850-β and B800-850-α genes Kiley, P. J.;S. Kaplan
  16. Microbiol. Rev. v.52 Molecular genetics of photosynthetic membrane biosynthesis in Rhodobacter sphaeroides Kiley, P. J.;S. Kaplan
  17. J. Microbiol. Biotechnol. v.7 Cloning, nucleotide sequence and expression of gene coding for polyhydroxybutyric acid (PHB) synthase of Rhodobacter sphaeroides 2.4.1 Kim, J. H.;J. K. Lee
  18. Appl. Environ. Microbiol. v.63 Polyhydroxyalkanoate production in Rhodfobacter capsulatus; genes, mutants, expression, and physiology Kranz, R. G.;K. K. Gabbert;T. A. Locke;M. T. Madigan
  19. Polymer Commun v.29 New bacterial polyesters produced in Alcaligenes eutrophus from organic acids Kunioka, M.;Y. Nakamura;Y. Doi
  20. J. Bacteriol. v.82 Assay of poly-β-hydroxybutyric acid Law, J. H.;R. A. Slepecky
  21. molecular Genetics of Photosynthesis: Frontiers in Molecular Biology Molecular genetics of purple bacteria Lee, J. K.;S. Kaplan;B. Anderson(ed.);A. H. Salter(ed.);J. Barber(ed.)
  22. J. Microbiol. Biotechnol. v.8 Characterization of a photosynthetic mutant selected by increased formation of poly-3-hydroxybutyrate in Rhodobacter spheroides Lee, I. H.;D. H. Kho;J. K. Lee
  23. J. Bacteriol. v.174 Isolation and characterization of trans-acting mutation involved in oxygen regulation of puc operon transcription in Rhodobacter sphaeroides Lee, J. K.;S. Kaplan
  24. Arch. Microbiol. v.155 Formation of poly(3-hydroxyalkanoates) by phototrophic and chemolithotrophic bacteria Liebergesell, M.;E. Hustede;A. Timm;A. Steinbichel;R. C. Fuller;R. W. Lenz
  25. J. Biol. Chem. v.253 Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodobacter sphaeroides Lueking, D. R.;R. T. Fralely;S. Kaplan
  26. J. Bacterol. v.176 Control of poly-β-hydroxybutyrate synthase mediated by acetyl phosphate in Cyanobacteria Miyake, M.;K. Kataoka;M. Shirai;Y. Asada
  27. J. Mol. Biol. v.147 Nucleotide sequence of the Kanamycin resistance transposon Tn903 Oka, A.;H. Stugisaki;M. Takanami
  28. Gene v.29 In vitro insertional mutagenesis with a selectable DNA fragment Prentki, P.;H. M. Krisch
  29. J. Biol. Chem. v.264 Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase (phbC) Peoples, O. P.;A. J. Sinskey
  30. J. Biol. Chem. v.264 Poly-βhydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding β-ketothiolase and acetoacetyl-CoA reductase Peoples, O. P.;A. J. Sinskey
  31. J. Microbiol. Biotechnol. v.9 Mass production of poly(3-hydroxybutyrate) by fed-batch cultures of Rastonia eutropha with nitrogen and phosphate limitation Ryu, H. W.;K. S. Cho;B. S. Kim;Y. K. Chang;H. J. Shim
  32. Molecular Cloning: A Laboratory Manual, (2nd ed.) Sambrook, J.;E. F. Fritsch;T. Maniatis
  33. Can. J. Microbiol. v.41 Intracellular poly(3-hydroxybutyrate) depolymerase in Alcaligenes eutrophus Saito, T.;K. Takizawa;H. Saegusa
  34. Biol. Technology v.1 A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram negative bacteria Simon, R.;V. Priefer;A. Puhler
  35. J. Gen. Microbiol. v.28 The kinetics of the synthesis of photopigments in Rhodopseudomonas sphaeroides Sistrom, W. R.