Identification of Yarrowia lipolytica Y103 and Its Degradability of Phenol and 4-Chlorophenol

  • Lee, Jeong-Soon (Department of Microbiology and Biotechnology, Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Kang, Eun-Jeong (Department of Microbiology and Biotechnology, Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Kim, Min-Ok (Department of Microbiology and Biotechnology, Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Lee, Dong-Hun (Department of Microbiology and Biotechnology, Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Bae, Kyung-Sook (Genetic Research Center, Korea Research Institute of Bioscience and Biotechnolgy) ;
  • Kim, Chi-Kyung (Department of Microbiology and Biotechnology, Research Institute for Genetic Engineering, Chungbuk National University)
  • Published : 2001.02.01

Abstract

A nonconventional yeast strain Y103 capable of degrading several aromatic hydrocarbons was isolated from the wastewater of the Yocheon industrial complex. The strain Y103 was identified as Yarrowia lipolytica on the basis of its unique dimorphic and biochemical characteristics as determined by a Biolog test. Y. lipolytica Y103 was found to degrade phenol and 4-chlorophenol to produce catechol. The catechol then will be further degraded to produce 2-hydroxymuconic semialdehyde via meta-cleavage. These results indicate that strain Y103 degrades 4-chlorophenol, phenol, and catechol through a consecutive reaction to produce 2-hydroxymuconic semialdehyde. The most active degradation of phenol by Y. lipolytica Y103 occurred with a 0.5 mM phenl concentration in an MM2 medium at $30^{\circ}C$ and pH 7.0.

Keywords

References

  1. J. Bacteriol. v.171 Regulator and enzyme specificities of the TOL plasmid-encoded upper pathway for degradation of aromatic hydrocabons and expansion of the substrate range of the pathway Abril, M. A.;C. Michan;K. N. Timmis;J. L. Ramos
  2. Appl. Environ. Microbiol. v.60 Formation of chlorocatechol meta-cleavage products by a Pseumonad during metabolism of monochlorobiphenyls Arensdorf, J. J.;D. D. Focht
  3. Nonconventional Yeasts in Biotechnology v.1 Yarrowia lipolitica Barth, G.;G. Gaillardin;K. Worf(ed.)
  4. FEMS Microbiol. Rev. v.19 Physiology and genetics of the dimorphic fungus Yarrowia lipolytica Barth, G.;G. Gaillardin
  5. Microbiology v.140 The lower pathway operon for benzoate catabolism in biphenyl-utilizing Pseudomonas sp. strain IC and the dioxygenase Carrington, B.;A. Lowe;L. E. Shaw;P. A. Williams
  6. Microbiol. Rev. v.55 Biodegradation of halogenated organic compounds Chaudhry, G. R.;S. Chapalamadugu
  7. Water Res. v.27 Accelerated phenol removal by amplifying the gene expression with a recombinant plasmid encoding catechol-2,3-dioxygenase Fujita, M.;M. Ike;T. Kamiya
  8. FEMS Microbiol. Rev. v.103 Microbial breakdown of halogenated aromatic pesticides and related compounds Haggblom, M. M.
  9. J. Bacteriol. v.171 Physically associated enzymes produce and metabolize 2-hydroxy-2,4-dienoate, a chemically unstable intermediate formed in catechol metabolism via meta cleavage in Pseudomonas putida Harayama, S.;M. Rekik;K. Ngai;L. N. Ornston
  10. J. Bacteriol. v.173 Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWWO plasmid xy/XYZ genes encoding benzoate dioxygenases Harayama, S.;M. Rekik;A. Bairoch;E. L. Neidle;L. N. Ornston
  11. J. Bacteriol. v.131 Component from the cell surface of the hydrocarbon utilizing yeast Candida tropicalis with possible relation to hydrocarbon transport Kappeli, O.;A. Fiechter
  12. J. Bacteriol. v.176 Nucleotide sequence and functional analysis of the meta cleavage pathway involved in biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp. strain KKS102 Kikuchi, Y.;Y. Ysukochi;Y. Nagata;M. Fukuda
  13. Kor. J. Microbiol. Biotechnol. v.24 Isolation and characterization of a phenol-degrading Candida tropicalis PW-51 Kim, S. B.;C. K. Kim;H. S. Kim;C. H. Lee;K. S. Shin;G. S. Kwon;B. D. Yoon;H. M. Oh
  14. Kor. J. Microbiol. v.35 Treatment of phenolic resin wastewater by Candida tropicalis PW-51 Kim, S. B.;C. K. Kim;H. S. Kim;B. D. Yoon;H. M. Oh
  15. J. Microbiol. v.37 Identification and characterization of an oil-degrading yeast, Yarrowia lipolytica 180 Kim, T. H.;J. H. Lee;Y. S. Oh;K. S. Bae;S. J. Kim
  16. J. Microbiol. Biotechnol. v.10 The possible involvement of the cell surface in aliphatic hydrocarbon utilization by an oil-degrading yeast, Yarrowia lipolytica 180 Kim, T. H.;Y. S. Oh;S. J. Kim
  17. J. Microbiol. v.35 Characteristics of catechol 2,3-dioxygenase produced by 4-chlorobenzoate degrading Pseudomonas sp. S-47 Kim, K. P.;D. I. Seo;K. H. Min;J. O. Ka;Y. K. Park;C. K. Kim
  18. J. Basic Microbiol. v.25 Degradation of phenol compounds by the yeast Candida tropocalis HP5 Krug, M.;H. Zieagler;G. Straube
  19. Appl. Microbiol. Biotechnol. v.49 Degradation of naphthalene by cells of Pseudomonas sp. strain NGK1 immobilized in alginate, agar, and polyacrylamide Manohar, S.;T. B. Karegouder
  20. J. Bacteriol. v.172 In vitro analysis of from Pseudomonas sp. strain CF600 Powlowski, J.;V. Shingler
  21. Biodegradation v.5 Genetics and biochemistry of phenol degradation by Pseudomonas sp. CF600 Powlowski, J.;V. Shingler
  22. Int. J. Food Microbiol. v.31 Growth of yeasts in milk and associated changes to milk composition Roostita, R.;G. H. Fleet
  23. J. Microbiol. Biotechnol. v.8 A pathway for 4-chlorobenzoate degradation by Pseudomonas sp. S-47 Seo, D. I.;J. C. Chae;K. P. Kim;Y. Kim;K. S. Lee;C. K. Kim
  24. J. Microbiol. v.35 Isolation of Pseudomonas sp. S-47 and its degradation of 4-chlorobenzoic acid Seo, D. I.;J. Y. Lim;Y. C. Kim;K. H. Min;C. K. Kim
  25. Biotechnol. Lett. v.12 Substrate inhibition of phenol oxidation by a strain of Candida tropicalis Stephenon, T.
  26. FEMS Microbiol. Lett. v.81 Cloning and expression of the Arthrobacter globiformis fcbA gene encoding dehalogenase (4-chlorobenzoate-4-hydroxylase) in Escherichia coli Tsoi, T. V.;G. M. Zaitsey;E. G. Plotnikova;I. A. Kosheleva;A. M. Boronin
  27. Handbook on Biodegradation and Biological Treatment of Hazardous Organic Compounds Van Agteren, M. H.;S. Keuning;D. B. Janssen
  28. J. Bacteriol. v.181 Evaluation of Acyl Coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica Wang, H. J.;M. T. Le Dall;Y. Wache;C. Laroche;J.-M. Belin;C. Gaillardin;J.-M. Nicaud