Effect of Plant Extracted Essential Oil on Antimicrobial Activity for Fish Phathogens

식물 추출 정유가 어류의 병인체에 미치는 항미생물 효과

  • 이근광 (나주대학 피부미용과) ;
  • 이정열 (군산대학교 해양생명과학부)
  • Published : 2001.06.01

Abstract

Antimicrobial activity of the essential oil extracted from plants Artemisia princeps var. orientalis, Thuja orientalis and Chamaecyparis obtusa were tested against selected pathogenic bacteria and fungi of fish. At the concentrations above 500ppm, ingibitory effect of the oil of A. princeps var. orientalis was at its peak against Aeromonas hydrophila ATCC 14715, A. hydrophila CF-2, A. salmonicida ATCC 14174 and A. salmonicida EL-1 but the bacteria Edwardsiella tarda KBF-1, Vibrio anguillarum ATCC19264, V. ordalii ATCC33509 and Streptococcus sp. were insensitive. Likewise, the oil extract of T. orientalis showed the highest inhibitory activity against V. ordalii ATCC33509, E. tarda ECK-1, and E. tarda KBF-1 at 300ppm; however the activity was highest at 500ppm or A. hydrophila ATCC14715, A. hydrophila CF-2, A. salomonicida ATCC14174, A. salmonicida EL-1 and Streptococcus sp. SF-1. With increasing dose of C. obtusa oil, the inhibitory activity became more and more effective against A. hydrophila CF-2, A. salomonicida ATCC14174, E. tarda ECK-1 and Streptococcus sp. SF-1, but A. hydrophila ATCC14174, A. salmonicida EL-1, E. tarda KBF-1, V. anguillarum ATCC19264, V. ardalii ATCC33509 and gram positive bacteria (Streptococcus sp.) were somewhat resistant. A. princeps var. orientalis, T. orientalis and C. obtusa were also tested against Saprolegnia sp. at the oil concentrations of 10, 100, 500, 1,000, 1,500 and 2,000ppm. The inhibitory effect of the oil on the inhibit the mycelial growth of Saprolegnia sp. at 10ppm and completely inhibited at over 500ppm.

Keywords

References

  1. J. Fish. Pathol. v.6 no.1 Drug resistance in fish pathogenic bacteria Aoki.T.
  2. J. Kor. Agric. Chem.Soc. v.33 no.2 Inhibitory effects of grapefruit seed extract(DF-100)on growth and toxin production of Penicillium islandicum Cho,S.W.;I.W.Seo;J.D.Choi;I.S.Joo
  3. Bull. Kor. Fish. Soc. v.23 no.4 Studies on the antimicrobial activity of grapefruit seed extract Choi,J.D.;I.W.Seo;S.H.Cho
  4. Eel.J. Vet. Serv. v.18 no.1 Studies on partial cgaracterization and pathogenicity of atypical A. salmonicida EL-1 isolated from diseased cultured Han,K.S.;I.Y.Choi;K.K.Lee
  5. Bull. Eur. Fish. Pathol. v.14 no.1 Isolation, Characterization and pathogenicity of a Streptococcus strain in the flounder cultured in Korea Kim,Y.G.;K.K.Lee
  6. Korean J. Vet. Serv. v.20 no.1 Studies on Edwardsielosis of cultured flounder Kim,Y.J.;K.S.Han;Y.G.Kim;K.K.Lee
  7. Kor. J. Mycol. v.27 no.1 Pathological characteristics of Saprolegnia sp. to snakehead and effect of essential oils to the growth of the water mold Lee,K.K.;Y.G.Kim;M.W.Lee;H.H.Lee
  8. J. Kum. Environ. Coll. v.1 Characteristics and pathogenicity of E. tarda L-1 isolated from cultured flounder and effect of control by plant essential oil Lee,K.K.;H.S.Kim;J.C.Lee;J.S.Kim;M.W.Lee
  9. Kor. Food soc. Technol. v.23 no.2 Antimicrobial effect of some plant extracts and their fractionates for food spoilage microorganisms Lee,B.W.;D.H.Shin
  10. Thin-layer chromatography(2nd ed.) Stahl,E.
  11. Kor. J. Ecol. v.15 no.1 Antifungalactibity of some essential oils against four fungi Yun,K.W.;N.K.Dubey;D.M.Han;B.S.Kil