배양한 흰주 대뇌세포에서 2,3,7,8-tetrachlorodibenzo-p-dioxin 이 2',3'-cyclic nucleotide 3'-phosphodiesterase(CNPase)의 표현에 미치는 영향

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase(CNPase) in rat cortical neurons in culture

  • 조선정 (경북대학교 자연과학대학 미생물학과) ;
  • 정재섭 (경북대학교 자연과학대학 미생물학과) ;
  • 김덕규 (동국대학교 의학연구소 신경과학연구부 및 의과대학 해부학교실) ;
  • 신승철 (동국대학교 의학연구소 신경과학연구부 및 의과대학 해부학교실) ;
  • 고옥 (동국대학교 의학연구소 신경과학연구부 및 의과대학 해부학교실) ;
  • 정용욱 (동국대학교 의학연구소 신경과학연구부 및 의과대학 해부학교실) ;
  • 고복현 (동국대학교 의학연구소 신경과학연구부 및 의과대학 해부학교실) ;
  • 진익렬 (경북대학교 자연과학대학 미생물학과) ;
  • 문일수 (동국대학교 의학연구소 신경과학연구부 및 의과대학 해부학교실)
  • Cho, Sun-Jung (Department of Anatomy and Research Institute for Natural Science of Dongguk University) ;
  • Jung, Jae-Seob (Department of Anatomy and Research Institute for Natural Science of Dongguk University) ;
  • Kim, Deock-Kyu (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Shin, Seung-Chul (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Go, Ok (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Jung, Yong-Wook (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Ko, Bok-Hyun (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Jin, Ing-Nyol (Department of Anatomy and Research Institute for Natural Science of Dongguk University) ;
  • Moon, Il-Soo (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
  • 발행 : 2001.08.01

초록

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) a prototype of the highly toxid halogenated arylhydrocarbons, bioaccumulates in the food chain and induces a complex spectrum of pathological responses. However, its effect on the nerve system is relatively not well studied. In this study we evaluated TCDDs cytotoxicity on the cortical cell and investigated its effect on the expression 2,3-cyclic nucleotide 3-phosphodiesterase(CNPase), a marker for oilgodendrocytes, The survival rates of 4 DIV cortical cells, that are dissociated from E18 rat cortex and maintained in the presence of TCDD, were 88.8, 83.6, 78.5, and 78.6%(5,10, 20 and 50 nM, respectively) where the reduction in 20 and 50mM TCDD were statistically very significant(p<0.01). Imunocytochemistry of cultured cells revealed that the intensities of immunostaining with an anti-CNP1&2 antibody depended on the concentrations of the toxin. Immunoblot analysis also showed differential expression of CNP1 and CNP2 in the presence of TCDD; the CNP1 expression was dose-dependently decreased. Interestingly, the expression of CNP2 in the presence if TDCC; the CNP1 expression was dose-dependently decreased. Interestingly, the expression of CNP2 fluctuated with the TCDD concentration. These results indicated that CNP1 and 2 are differentially regulated by TCDD, implying the functions of oligodendrocytes are modulated by the toxin.

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