한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제29권1호
  • /
  • Pages.25-30
  • /
  • 2001
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Cathepsin B 저해물질을 생산하는 Streptomyces misakinesis의 동정 및 저해물질의 분리

Identification of Streptomyces misakiensis Producing Cathepsin B Inhibitor and the Purification of Inhibitor

  • 한길환 (영남대학교 응용미생물학과) ;
  • 김상달 (영남대학교 응용미생물학과)
  • 발행 : 2001.03.01
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초록

A strain of Actinomycetes producing cathepis B inhibitor was isolated from soil and identified as Streptomyces misakiensis. The product of S. misakiensis inhibited effectively cathepsis B proteinases as well as trypsin and papain. The cathepsin B inhibitor were largely produced with incubation for 4 days. The S. misakiensis was the most growth with incubation for 5 days. The cathepsin B inhibitor was isolated from the extraction of both with ethanol, ethanol and chlorofrom, and following several column chromatography such as sephadex G-15, silica gel 60 and sephadex LH-20 chromatography. The moleculer weight of purfied inhibitor was 138 dalton.

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