동아시아식생활학회지 (Journal of the East Asian Society of Dietary Life)

  • 제11권1호
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  • Pages.26-32
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  • 2001
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  • 1225-6781(pISSN)
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  • 2288-8802(eISSN)
동아시아식생활학회 (The East Asian Society of Dietary Life)
권호 표지

독소원성 대장균과 Clostridium perfringens A형이 생산하는 장독소의 검색을 위한 RPLA 법과 PCR기법의 감도 비교

Comparison of Sensitivity of Detection for Enterotoxigenic Escherichia coli Enterotoxin and Clostridium perfringens Type A Enterotoxin by Means of the Reversed Passive Latex Agglutination and the Polymerase Chain Reaction

  • 발행 : 2001.02.01
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초록

독소원성 대장균(enterotoxigenic Escherichia coli, ETEC, EC81, serotype O148:H28)이 생산하는 heat labile enterotoxin(LT)를 검색해 본 결과, reversed passive latex agglutination(RPLA)법에 있어서는 2배로 희석한 용액 (50 ng)으로부터 64로 희석한 용액 (1.56 ng)에서까지 양성반응을 보였으며 polymerase chain reaction (PCR)기법에 있어서는 10 ng으로부터 1 pg희석용액에서까지 147-base pair(bp)의 LT DNA fragment가 확인되었다. Clostridium perfringens A형 (NCTC8238, Hobbs serotype 2)이 생산한는 장독소를 검색해 본 결과, RPLA법에 있어서는 2배로 희석한 용액 (50 ng)으로부터 64로 희석한 용액 (1.56 ng)에서 까지 양성반응을 보여 독소원성대장균이 생산하는 LT와 일치하였으나, PCR기법에 있어서는 10ng로부터 10 pg 희석용액에서 까지 354-bp의 DNA fragment가 확인되어 독소원성대장균이 생산하는 LT보다 1/10의 낮은 감도를 보였다. PCR기법은 RPLA법에 비하여 훨씬 신속하고 소량의 sample로 장독소를 확인할 수 있었다.

키워드

참고문헌

  1. Int. Immunol. v.21 Biological evaluation of a methanol soluble, heat-stable Escherichia coli enterotoxin in infant mice, pig, rabbits, and calves Burgess, M. N.;Bywater, R .J.;Cowley,J. M.;Mullen, N. A.;Newsome, P. M.
  2. J. Biol. Chem. v.256 Amino acid sequence of heat-stable enterotoxin produced by Escherichia coli pathogenic for man Chan, S.;Giannella, R. A.
  3. J. Clin. Microbiol. v.28 Comparison of three assay systems for detection of enterotoxigenic Escherichia coil heat=stable enterotoxin Cryan, B.
  4. Eur. J. Clin. Microbiol. Int. Dis. v.9 Comparison of the synthetic oilgonucleotide gene probe and infant mouse bioassay for detection of enterotoxigenic Escherichia coil Cryan, B.
  5. J. Bacteriol. v.139 Cistrons encoding Escherichia coil heat-labile toxin Dallas, W. S.;Gill, D. M.;Falkow, S.
  6. Antonie Van Leeuwenhock v.56 Cloning and sequencing of the Clostridium perfringens enterotoxin gene Damme-Jongsten, M. V.;Wernars, K.;Notermans, S.
  7. Bacteriology product information Denka Seiken
  8. Appl. Microbiol. v.16 Improved medium for sporulation of Clostridium perfringens Duncan, C. L.;Strong, D. H.
  9. EMBO J. v.5 Tetanus toxin: Primary structure, expression in Escherichia coli, and homology with botulinum toxins Eisel, U.;Jarusch, W.;Goretzki, K.;Hanschen, A.;Engels, J.;Weller, U.;Hudel, M.;Habermann, E.;Niemann, H.
  10. Inf. Imminol. v.14 Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin : Characteristics of the model Giannella, R. A.
  11. Pharmacol. Ther. v.11 Escherichia coli heat-stable enterotoxin Greenberg, R. N.;Guerrant, R. L.
  12. FEMS MIcrobiol. Letters v.108 Amino acid sequence of heat-labile enterotoxin from chicken enterotoxigenic Escherichia coli is identical to that of human strain H10407 Inoue, T.;Tsuji, T.;Koto, M.;Imamura, S.;Muyama, A.
  13. J. Jpn. Assoc. Inf. DIs. v.53 Assay for enterotoxin n fecal specimens of Clostridium perfringens food poisoning Itoh, T.;Inaba, M.;Saito, K.;Sakai, S.;Uemura, T.;Sakaguch, G.
  14. Kor. J Env. Hlth. Soc. v.23 Comparison of sensitivity of detection for Clostridium perfringens type A enterotoxin by use of the reversed passive latex agglutination and polymerase chain reaction Jung, H. K.
  15. Am. J. Vet. Res. v.60 Identification of serotype by use of serologic assay and detection of the enterotoxin gene of Escherichia coli by means of a polymerase chain reaction assay for isolates from pigs, chickens, and cows Jung, H. K.
  16. Kor. J. Env. Hlth. Soc. v.25 Comparison of sensitivity of detection for enterotoxigenic Escherichia coli enterotoxin by use of the reversed passive latex agglutination and the polymerase chain reaction Jung, H. K.
  17. Ann. Rep. Tokyo Metr. Res. Lab. Publ. Hlth. v.33 Prevalance of spore-forming bacteria in sugar, starch, spices, and vegetable protein Kokubo, Y.;Jinbo, K.;Murakami, F.;Murakami, H.
  18. Ann. Rev. Microbiol. v.17 New approaches to bacterial taxonomy. Marmur, J.;Falkow, S.;Mendel, M.
  19. Adv. Vet. Sci. Comp. Med. v.18 Pathogenesis of enteric diseases caused by Escherichia coli Moon, H. W.
  20. Removal of polysaccharides from existing genomic DNA preps. Murray, R. G. E.;Thompson, B. G.
  21. Short protocols in molecular biology Ausubel, F. M.;Brent, R.;Kingston, R. E.;Moore, D. D.;Seidman, J. G.;Smith, J. A.;Struhl, K.(eds.)
  22. J. Clin. Microbiol. v.18 Identification of enterotoxigenic Escherichia coli isolated from swine with diarrhoea in Thailand by colony hybridization, using three enterotoxin gene probes Patamoroj, U.;Seriwatana, J.;Echeverria, P.
  23. FASEB J. v.155 Structure of a heat-stable enterotoxin produced by a human strain of Escherichia coli Ronnberg, B.;WAdstrom, T.;Jernvall, H.
  24. Ann. REv. Microbiol. v.29 Human diarrheal disease caused by enterotoxigenic Escherichia coli Sack, R. B.
  25. Int. J. Food Microbiol. v.17 Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure Saito, M.;Matsumoto, M.;Funabashi, M.
  26. J. Med. Microbiol. v.3 The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine organ Smith, H. W.;Gyles, C. L.
  27. J. Jpn. Publ. Hlth. v.28 Detection of strain and enterotoxin in fecal specimens of Clostridium perfringens food poisoning Tsukamoto, T.;ishibashi, M.;Asao, T.;Ohtsu, K.;Shinagawa, K.;Kunito, D.;Uemura, T.
  28. J. Appl. Bacteriol. v.44 Incidence of enterotoxigenic Clostridium perfringens in healthy humans in relation to the enhancement of enterotoxin production by heat-treatment Uemura, T.
  29. Vet. Microbiol. v.22 DNA probes for the detection of toxin genes in Escherichia coli isolated from diarrhoeal disease in cattle and pigs Woodward, M. J.;Kearsley, R.;Wray, C.(et al.)