Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Cultivation of Lactobacillus crispatus KLB46 Isolated from Human Vagina

  • Chang, Chung-Eun (Department of Biological Engineering, Inha University) ;
  • Kim, Seung-Cheol (Department of Obsterics and Gynecology, College of Medicine, Ewha Womans University) ;
  • So, Jae-Seong (Department of Biological Engineering, Inha University) ;
  • Yun, Hyun-Shik (Department of Biological Engineering, Inha University)
  • Published : 2001.03.01
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Abstract

Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a string antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63h(sup)-1 and the highest viable cell count (1.9$\times$10(sup)9 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53$\times$10(sup)9 CFU/mL) was obtained at a dilution rate of 0.32h(sup)-1, and was 7.33$\times$10(sup)11 CFU L(sup)-1 h(sup)-1, that is approximately 5 times higher than that obtained from batch culture.

Keywords

References

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