KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Effects of Green and Taste Teas on the Growth and Vacuolating Toxin Titer of Helicobacter pylori

녹차 등의 기호차가 Helicobacter pylori의 증식 억제와 공포화 독소 역가에 미치는 영향

  • 정양숙 (고신대학교 의과대학 미생물학교실) ;
  • 강경희 (고신대학교 의과대학 미생물학교실) ;
  • 장명웅 (고신대학교 의과대학 미생물학교실)
  • Published : 2001.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to evaluate the effects of green and taste teas on the in-vitro antimicrobial activity and vacuolating toxin titer of Helicobacter pylori. Crude aqueous extracts prepared by adding 2 g of tea leaf or powder to 100 ml of boiling distilled water, and sterilized by passing through a 0.22 $mutextrm{m}$ membrane filter. Green tea, coffee, and ginger tea showed bactericidal activity on H. pylori within 3 hours. Black tea and ssangwha tea also showed bactericidal activity on H. pylori in 24 hours. Arrowroot tea show no bactericidal effect on H. pylori after 48 hours. Two fold diluted green tea and coffee decreased(1/10,000cfu) the growth of H. pylori in 24 hours, but the two fold diluted black tea, ssangwha tea, and ginger tea showed suppression effect upon of(1/10cfu) H. pylori in 24 hours. The two-fold and 10-fold diluted green tea, coffee and two-fold diluted black tea abrogated the vacuolating toxin titer of H. pylori, but the two-fold and 10-fold diluted ginger, ssangwha, ginseng, and arrowroot tea only reduced the vacuolating toxin titer of H.pylori from 1/2 to 1/8. These result suggest that green tea and coffee have effective antibacterial or bactericidal effects on H.pylori, and that they also have a neutralization effect upon the vacuolating toxin of H.pylori.

Keywords

References

  1. Lancet v.1 Unidentified curved bacilli on gastric epithelium in active chronic gastritis Marshall, B. J.;Warren, J. R.
  2. New Engl. J. Med. v.325 Helicobacter pylori infection and gastric cancer among Japanese Americans in Hawaii Nomura, A.;Stemmerman, G. N.;Chyou, P. H.
  3. Microbiol. Immunol. v.44 no.5 Helicobacter pylori may be have only a transient presence in the oral cavity and on the surface of oral cancer Okuda, K.;Ishihara, K.;Miura, T.;Katakura, A.;Noma, H.;Ebihara, Y.
  4. v.343 Identification of Helicobacter pylori in gastric specimens, gastric juice, saliva, and feces of Japanese patients, Lancet Shmada, T.;Ogura, K.;Ota, S.
  5. Korean J. Int. Med. v.14 Changes in the evolution of the antigenic profiles and morphology during coccoid conversion of Helicobacter pylori Roe, I. M.;son, S. H.;Oh H. T.;Choi, J.;Shin, J. H.;Lee, J. W.;Hah, Y. C.
  6. Ann. Intern. Med. v.116 Effect of treatment of Helicobacter pylori infection on the long-term recurrence of gastric or duodenal ulcer Graharm, D. Y.;Lew, G. M.;Klein, P. D.
  7. Gastroenterol. v.102 Factors influencing the eradication of Helicobacter pylori with triple therapy Graham, D. Y.;Lew, G. M.;Malaty, H. M.;Adam, E.;Klein, P. D.
  8. J. Clin. Microbiol. v.34 Metronidazole and clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents in color indicator egg york agar in a miniwill format Vasquez, A.;Valdez, Y.;Giman, R. H.
  9. J. Clin. Microbiol. v.35 Comparative evaluation of the E test, agar dilution, and broth microdilution of testing susceptibilities of Helicobacter pylori strains to 20 antimicrobial agents Piccolomini, R.;Bonaventura, G.;Catmo, G.
  10. Gastroenterol. v.112 Antimicrobilal susceptibility of Helicobacter pylori in Canada to three key antiviotics: metronidazole, clarithromycin, and amoxacillin Fedorak, R.;Archambault, A.;Flamm, R.;Osta M.;Stmler, D.
  11. H. pylori strains v.34 no.6 Antibiotic susceptibility of Helicobacter pylori and the combination effect of antibioties on the antibiotic-resistant Song, G. Y.;Chang, M. W.
  12. Antimicrob. Agents Chemother. v.39 Antimicrobial properties of Tea(Camellia sinensis L) Hamilton-Miller,;J. M. T.
  13. Science and culture of green tea Classification of green tea Kim, T. J.;Kim T. J.(Eds.)
  14. J. Agrc. Food. Chem. v.40 Antimicrobial activity of green tea flavor components and their combination effects Kubo, I.;Muroi, H.;Himejimma, M.
  15. Science and culture of green tea Functional components of green tea Kim, T. J.;Kim T. J.(Eds.)
  16. J. Sci. Food Agrc. v.26 The nutritional and therapeutic value of tea- a review Stagg, G. V.;Millin, D. J.
  17. Biochem. Biophys. Acta v.1127 Tea catechins decrease micellar solubility and intestinal adsorption of cholesterol in rats Ikeda, I;Imasato Y.;Sasaki, E.;Nakayama, M.;Nagao, H.;Takeo, T.;Yayabe, F.;Sugano, M.
  18. Pre. Med. v.21 Suppression of genotocicity of carcinogens by epigallocatechin gallate Hayatsu, H.;Inada, N.;Kakutani, T.;Arimoto, S.;Negishi, T,;Mori, K.;Okuda, T.;Sakata, I.
  19. Jap. J Agr. Chem. v.61 Angiotesin I reductase inhibitor activity of green tea Hara Y.;Matsusaki M.;Sasaki K.
  20. Jap. J Agr. Chem. v.59 Anti-oxidant activity of green tea catechins Matsusaki, M.;Hara, Y.
  21. J. Jpn. Assoc. Infect. Dis. v.66 Antimicrobial and microbicidal activities of tea and catechins aganist Mycoplasma Chosa, H.;Toda, M.;Okubo, S.;Hara, Y.;Shimamura, T.
  22. Nutr. Bull v.29 Tea-the elements of a cuppa Stagg, G. V.
  23. J. Bacteriol v.45 Antibacterial and anti-hemolysin activities of tea catechins and their structrual relatives Toda, M.;Okubo, S.;Ikigai, H.;Shimamura, T.;
  24. Nippon Shokuhin Kogyo Gakkaishi v.36 Antibacterial activities of tea polyphenols against foodborne pathogenic bacteria Hara, Y.;Ishigami, T.
  25. FEMS Microbiol. Let. v.152 Microbiological activity of whole and fractionated crude extracts of tea(Camellia sinensis), and of tea componentss. Yam, T. S.;Shah, S.;Hamilton-Miller;J. M. T.
  26. Letters in Appl. Microbiol v.8 The bactericidal activity of tea and coffee. Toda, M.;Okubo, S.;Hiyoshi, R.;Shimamura, T.
  27. Antimicrob. Agents Chemother v.43 In vitro and in vivo activities of tea catechins against Helicobacter pylori Mabe, K.;Yamada, M.;Oguni, I.;Takahashi, T.
  28. J. Korean Soc. Microbiol v.32 Effect of ginseng extracts on the production of vacuolating toxin by Helicobacter pylori Change, M. W.;Song, G. Y.;Cha, H. S.
  29. Eur. J. Epidemiol. v.9 Evaluation of rapid urease test for detection of Helicobacter pylori in gastric biopsy specimens Kamiya, S.;Taniguchi, T.;Yamomoto, T.;Shirai, T.;Harasawa, S.;Miwa, T.;Ozawa, A.
  30. Eur. J. Gastroenterol. Hepatol. v.6 Characteristics of vacuolating toxin produced by Helicobacter pylori. Kamiya, S.;Kai, M.;Ozawa, A.;Kobayashi, H.;Shirai, T.;Harasawa, S.;Miwa, T.
  31. Mutation Res. v.210 Crude tea extracts decrease the mutagenic activity of N-methyl-N'-nitro-N-nitroso-guanidine in vitro and intrgastric tract or rats Jain, A. K.;Shimoi,K. Nakamura, K.;Kada, Y.;Hara T.;Tomita, I.
  32. Jap. J. Bacteriol. v.55 Cenomic structure of Helicobacter pylori and its pathogenicity Kamiya K.
  33. Biochem Biophys v.1147 Bactericidal catechins damage the lipid bilayer Ikigai H.;Nakae T.;Hara Y,;Shimamura T
  34. Letters in Appl Microbiol v.9 The anti-haemolysin activity of tea and coffee Okubo S.;Ikigai H.;Toda M.;Shimamura T.