Screening for Antioxidant Activity of Edible Plants

식용식물의 항산화효과 검색

  • 조세연 (고려대학교 가정학과) ;
  • 한용봉 (고려대학교 가정학과) ;
  • 신국현 (서울대학교 천연물과학연구소)
  • Published : 2001.02.01

Abstract

The antioxidant activitiesof methanol extracts of twenty one vegetables were tested using the method of 1,1-diphenyl-2-picryl hydrazyl (DPPH) reactivity and TBARS substance assay in vitro. The methanol extracts of the leaves from three plants such as Aster scaber, Chrysanthemum coronarium, Petasites japonicus were found to be th most effective on DPPH radical scavenging activity. The next effective ones were the leaves of Capsicum annuum, the peels of Ipomoea batatas and Solanum tuberosum, the stems and leaves of Ipomoea batatas, the levels of Solanum melongena, the fruits of Solanum melongena, the green fruits (seed) of Capsicum ammuum in order, and the others did not show a considerable activity. When tested on lipid peroxidation using a lipid peroxidation generation system mediated by $H_{2}O_{2}/Fe^{2+}$ in rat liver homogenates. the peels of Cucumis melo, the leaves and the fruits of Solanum melongena, of Solanum melongena, The levels of Aster scaber and Chrysanthemum coronarium, the stems and leaves of Ipomoea batatas and the peels of Ipomoea batatas were found to exhibit a good protective activity against lipid peroxidatian in the order of effectiveness.

21종 식용식물의 부위별 MeOH Ex. DPPH와 TBA법으로 항산화 효과를 조사한 결과 DPPH를 이용한 유리라디칼 소거 효과는 참취잎, 쑥갓잎 및 머위잎에서 특이적으로 높은 효과가 관찰되었으며 시료추출물의 buffer에 의해 유발된 과산화지질 억제효과는 참외껍질의 억제 효과 강도는 $IC_{50}$ 값이 $10\mu\textrm{g}/mL$로 매우 강하였다. $S_2O_z$로 유발시킨 지질과산화에 대한 억제효과는 0.25 mg 농도에서 L-ascorbic acid의 과산화 억제율과 비교하면 가지 잎, 머위 잎, 감자껍질의 항산화 억제 효과가 가장 높았으며 $FeSO_4$로 유발시킨 지질과산화에 대한 억제효과는 쑥갓잎의 $IC_{50}$가 0.08 mg/mL로 특이적으로 높았다.

Keywords

References

  1. Bio Chem. J. v.219 Oxygen toxicity, oxigen radicals, transition metas and disease Halliwell B.;Gutteridge J, M, C,
  2. Physiol. Rev. v.59 Hydroperoxide metabolism in mammalian organs Chance B.;Sies H.;Boveris A.
  3. 대우학술총서 노화 김숙희;김화영
  4. Ann. Inter. Med. v.107 Oxidative DNA damage,cancer and aging. Oxygen and human disease Ames B. N.;Saul R. L.
  5. M. S. Thesis Antioxidative activity and pharmaco-constituents of Houttuyniae herba Kim J. H.
  6. Ph. D. Dissertation Studies on biological activities of Spargnanium erectum Shin U. Y.
  7. Free Radic. Bio. Med. v.15 Current status of antioxidant therapy Rice-evans C. A.;Diplock A. T.
  8. Korean J. Food Sci. Technol. v.27 Antioxidative activity and physilological activity of some Korean medicinal Kim H. K.;Kim Y. E.;Do J. R.;Lee Y. C.
  9. Korean J. Food Sci. Technol. v.24 Synergistic effect of Rhus javanica Linne ethanol extract containing several synergist Chang Y. S.;Choi U.;Shin D. H.;Shin J. I.
  10. Korean J. Food Sci. Technol. v.24 Screening of natural antioxidant from plant and their antioxidative effect Choi U.;Shin D. H.;Chang Y. S.;Shin J. I.
  11. Korean J. Appl. Microbiol. Bioeng. v.9 Antioxidant properties of red-pepper peel extracts on margarine Yu J. H.;Cho C. M.;Oh D. H.
  12. Natural Medicines v.51 Antiperoxidation activity of traditional chinese prescriptions and their main crude drugs in vitro Yokozawa T.;Dong E.;Liu Z. W.;Oura H.
  13. J. Agric. Food. Chem. v.44 Total antioxidant capacity of fruits Wang H. Cao G.;Prior R. L.
  14. Cancer Letters v.114 Flavonoids as bioactive components in apple products Van Der Sluis A. A.;Dekker M.;Jongeun W. M. F.
  15. Special Presentation. Korean Soc. Food Sci. Technol. Antioxidative activity of anthocyanin pigments in black rice seeds Choi S. W.;Osawa T.;Kawacishi S.Tashiro T.
  16. J. Korea Soc. Food Nutr. v.23 Further screening for antioxidant activity vegetable plants and its active principles from Zanthoxylum schinifolium Mun S. I.;Ryu H. S.;Lee H. J.;Choi J. S.
  17. M. S. Thesis. Studies on biological activities of Petasites japonicus Lee M. O.
  18. Korean J. Food Sci. Technol. v.31 Characteristics of antioxidative and antimicrobial activities of various cultivars of sweet potato Lee H. H.;Kang S. G.;Rhim J. W.
  19. Kor. J. Pharmacogen. v.24 Isolation of steriods and flavonoids from the herb of Oenanthe javanica Dc Park J. C.;Yu Y. B.;Lee J. H.