생명과학회지 (Journal of Life Science)

  • 제11권1호
  • /
  • Pages.19-23
  • /
  • 2001
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

효모 탐색을 위한 Pyrolysis Mass Spectrometry의 활용

Application of Pyrolysis Mass Spectrometry on Yeast Screening

  • 신기선 (생명공학연구소 유전자은행) ;
  • 신용국 (조선대학교 단백질소재연구센터) ;
  • 권오유 (충남대학교 의과대학 해부학교실) ;
  • 이상한 (생명공학연구소 첨단생물소재연구센터)
  • 발행 : 2001.02.01
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초록

To develop the effective microbial screening method, pyrolysis mass spectrometry (PyMS) fingerprinting was evaluated as a tool that discriminate various yeast strains. The target yeast strains were isolated from industrial wastewater. Seventeen environmental isolated yeast strains were examined by pyrolysis mass spectrometry and sequencing analysis of the large subunit rRNA gene D1/D2 region. The PyMS results were compared with those of sequencing analysis. Taxonomic correlations were observed between the PyMS data and the sequencing results. It was concluded that PyMS provides a rapid, reliable and cost-reducing method for discrimination of the yeast strains.

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