한국균학회지 (The Korean Journal of Mycology)

  • 제29권1호
  • /
  • Pages.61-66
  • /
  • 2001
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지

Cordyceps militaris로부터 분리한 Ergosterol Peroxide의 한국인 암세포주에 대한 항암작용

In vitro Antitumor Activity of Ergosterol Peroxide Isolated from Cordyceps militaris on Cancer Cell Lines from Korean Patients

  • 김하원 (서울시립대학교 생명과학과) ;
  • 김영호 (충남대학교 약학대학) ;
  • 채흥복 (충남대학교 약학대학) ;
  • 남경숙 (서울시립대학교 생명과학과) ;
  • 이승정 (서울시립대학교 생명과학과) ;
  • 안혜숙 (서울시립대학교 생명과학과) ;
  • 정은호 (서울시립대학교 생명과학과) ;
  • 윤승현 (서울시립대학교 생명과학과) ;
  • 성수경 (서울시립대학교 생명과학과) ;
  • 이성진 (서울시립대학교 생명과학과) ;
  • 현진원 (서울대학교 의과대학 약리학교실)
  • Kim, Ha-Won (Department of Life Science, University of Seoul) ;
  • Kim, Young-Ho (College of Pharmacy, Chung Nam National University) ;
  • Cai, Xing Fu (College of Pharmacy, Chung Nam National University) ;
  • Nam, Kyong-Suk (Department of Life Science, University of Seoul) ;
  • Lee, Seung-Jung (Department of Life Science, University of Seoul) ;
  • An, Hye-Suk (Department of Life Science, University of Seoul) ;
  • Jeong, Eun-Ho (Department of Life Science, University of Seoul) ;
  • Yun, Seung-Hyun (Department of Life Science, University of Seoul) ;
  • Sung, Su-Kyong (Department of Life Science, University of Seoul) ;
  • Lee, Sung-Jin (Department of Life Science, University of Seoul) ;
  • Hyun, Jin-Won (Department of Pharmacology, College of Medicine, Seoul National University)
  • 발행 : 2001.04.30
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초록

곤충기생성 균류인 번데기동충하초는 각종 약리활성을 나타내는 것으로 유명하다. 국내에서 인공재배한 번데기동충하초 자실체를 n-hexane으로 추출하여 항암성분을 실리카젤 칼럼크로마토그래피로 순수하게 분리하여 $^1H-NMR$과 $^{13}C-NMR$로 그 구조를 밝혀본 결과 ergosterol peroxide $(5{\alpha},\;8{\alpha}-epidioxy-24(R)-methylcholesta-6,22-dien-3{\beta}-ol)$로 밝혀졌다. 분리한 crgosterol peroxide를 한국인의 암환자에서 분리한 각종 암세포에 대하여 항암작용을 측정한 결과, 3일 후에 위암세포주인 SNU-1 암세포에 대하여 가장 강한 항암작용을 나타내었다. 한국인의 암환자에서 유래한 위암세포인 SNU-1, 간암세포인 SNU-354 및 직장암세포인 SNU-C4 암세포 등에 대한 6일 후에 ergosterol peroxide의 50% 성장억제농도는 각각 75.8, 39.7, $32.7{\mu}g/ml$이었다. 따라서 ergosterol peroxide 성분은 번데기동충하초의 항암성분중의 하나로 밝혀졌다.

Entomopathogenic fungus of Cordyceps militaris is famous of its medicinal efficacies. An antitumor compound was purified from the n-hexane extract of artificially cultivated fruiting bodies of C. militaris and identified as ergosterol peroxide $(5{\alpha},\;8{\alpha}-epidioxy-24(R)-methylcholesta-6,22-dien-3{\beta}-o1,\; C_{28}H_{44}O_3)$ mainly by $^1H\;and\;^{13}C-NMR$ spectroscopic techniques. When the antitumor activity of ergosterol peroxide was measured against 3 tumor cell lines from Korean cancer patients, it showed the most strong activity against gastric cancer SNU-l cell line 3 days after treatment. The 50% inhibitory concentrations $(IC_{50})$ of ergosterol peroxide 6 days after treatment were $75.8{\mu}g/ml$ for human gastric SNU-1 tumor cell line, $39.7{\mu}g/ml$ for human colorectal SNU-C4 tumor cell line and $32.7{\mu}g/ml$ for human hepatoma SNU-354 cell line.

키워드

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