The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Analysis of Genetic Relationship of Entomogenous Fungi in Korea by Morphological Characteristics and RAPD

한국에서 채집한 Entomogenous fungi의 형태와 RAPD에 의한 유연관계 분석

  • 최인영 (전라북도 농업기술원) ;
  • 최정식 (전라북도 농업기술원) ;
  • 유영진 (전라북도 농업기술원) ;
  • 이왕휴 (전북대학교 농생물학과(농업과학기술연구소))
  • Published : 2001.04.30
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Abstract

RAPD test and the observation of morphological, cultural characteristics of fourteen selected entomogenous fungi were conducted to investigate the analysis of their internal relationships. Paecilomyces tenuipes showed snow flower form attached to numerous white conidiophores, produced globular and semi-egg types on the club types of phialides. Cordyceps militaris formed globosely conidiophores, dark yellow fruiting body on pupa. The phialide as on Acremonium-type in global conidiophores. Beauveria bassiana covered with conidia was not formed fruiting body and adhered conidia on conidiophore of zigzag type. The PDA and SDAY medium were confirmed as an optimum growth of them. P. tenuipes showed to velvet and plane types in several media whereas C. militaris was belong to centrally raised and floccose in the morphological type. In contrast, B. bassiana covered with conidia on velvet shape. The size of amplified products were analyzed by RAPD using URP primer and were from 100 bp to 2.0 kb with $10{\sim}14$ geuomic DNA. Total similarities of two groups were by dendrogram of UPGMA analysis. The homology of P. tenuipes groups was 94.8 to 100%. It also showed 70.1 to 96.6% in C. militaris group and B. bassiana was higher similarity than any other. The internal change of C. militaris, produced telemorph fruiting body, was higher seperated in species than P. tenuipes and B. bassiana in the RAPD.

Entomogenous fungi의 수집된 14균주를 대상으로 종내 그룹의 상호 유연관계를 분석하고자 자실체 및 불완전세대의 형태적, 배양적 특징 관찰과 RAPD 검정을 실시하였다. Paecilomyces tenuipes는 수 없이 많은 흰색 분생포자가 자실체에 불어 눈꽃모양을 나타냈으며, 곤봉형 phialides위에 구형 또는 타원형의 분생포자가 연쇄상을 이루었다. Cordyceps militaris는 담황색 자실체가 방망이 모양으로 번데기에서 형성되었으며, 불완전세대는 Verticillium으로 단생의 phialides에 구형의 분생포자가 Acremonium-type으로 부착되어 있었다. Beauveria bassiana는 자루에서 여러마디가 이어지며, 각 마디에서 1개씩 분생포자가 붙어있는 zigzag type이었다. 또한 이들의 배양에 적합한 배지는 PDA, SDAY이며, 평면과 단면구조로 Paecilomyces는 벨벳과 평탄, Cordyceps는 양모상과 중앙융기로 생육하였고, Beauveria는 벨벳에 밀가루를 뿌린 모양이었다. URP primer를 이용한 rDNA의 RAPD분석 결과 증폭된 산물의 크기는 100bp에서 2.0kb사이로 genomic DNA fragment는 $10{\sim}14$개이었다. UPGMA 분석에 의한 dendrogram 작성 결과 다양한 유전적 분화를 나타내었으며, 2개의 군으로 그룹화 하였다. P. tenuipes 그룹의 $94.8{\sim}100%$, C. militaris와 C. scarabaeicola, B. bassiana 그룹은 $54.3{\sim}$100%의 상동성을 보였다. 또한 완전세대의 자실체를 형성하는 C. militaris 그룹은 불완전세대인 P. tenuipes와 B. bassiana보다 종내의 변화의 폭이 컸으며, 종내의 세분화가 이루어졌다.

Keywords

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