Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Regenerative Effects of Houttuynia cordata Extracts against Cadmium-induced Cytotoxicity in NIH 3T3 fibroblasts Cultures (VII)

카드뮴 독성으로 손상된 NIH 3T3 섬유모세포에 대한 어성초 추출물의 재생효과(VII)

  • Lee Jeong-Ho (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Oh Hyun-Ju (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Jeong Seung-Il (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chun Hyun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Han Do-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Lee Hyun-Ok (Department of Dental Hygiene, Wonkwang Health College) ;
  • Ahn Jong-Woong (Korea Institute of Chemical Research, Natural Products Research Team) ;
  • Lee Mee-Hee (Osio Oriental Medicine) ;
  • Baek Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 이정호 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 오현주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 정승일 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 천현자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 이현옥 (원광보건대학 치위생과) ;
  • 안종웅 (화학연구원, 화학물질연구팀) ;
  • 이미희 (오시오 한의원) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2001.12.29
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Abstract

This study was carried out to evaluate antitoxic effects of Houttuynia cordata extract on cadmium by colorimetric methods. The antitoxic activity ofc in NIH 3T3 fibroblasts was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treated cells. The concentration of 10-2 mg/ml of Houttuynia cordata extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were antitoxic and tend to regenerate. These results suggest that the ethyl acetate extract of Houttuynia cordata retains a potential antitoxic activity.

Keywords

References

  1. 본초강목 v.하 이시진
  2. Chem. Pharm. Bull v.36 no.5 Platelet aggregation inhibitors from Jyu-yaku (Houttuyniae Herb) Nishiya, H.;Ishiwata, K.;Komatsu, K.;Nakata, O.;Kitamura, K.;Fujh, S.
  3. Planta Med v.61 Virucidal effects of the steam Distillate from Houttuynia cordata and its components on HSV-1, influenza virus, and HIV Hayashi, K.;Kamiya, M.;Hayashi, T.
  4. Planta Med v.58 Aristolactams and a 4,5-dioxoaporphine derivative from Houttuynia cordata Probstle, A.;Bauer, A.
  5. Planta Med v.56 In vitro Regeneration of Houttuynia cordata: A medicinal herd Borthakur, M.;Singh, R.S.;Bora, P.
  6. 대한예방한의학회지 v.4 no.1 어성초 추출물의 세포독성과 항균효과(IV) 이정호;박낭규;양은영;이현옥;한동민;백승화
  7. 한국식품과학회지 v.29 no.3 어성초의 화학성분 및 항미생물 활성 김근영;정동욱;정희종
  8. Kor. J. Pharm. Korea v.44 The inhibitory effects of Houttuynia cordata THUMB against cadmium induced cytotoxicity(II) Lee, J.H.;You, I.S.;Kim, J.S.;Lee, K.N.;Chung, W.Y.;Han, D.S.;Beak, S.H.
  9. J. Immunol. Methods. v.65 Rapid colorimetric assays for cellualr growth and survival: application to proliferation and cytotoxicity assays Mosmann, T.
  10. J. Natl. Cancer Inst. v.82 New colorimetric cytotoxicity assay for anticancer-drug screening Skehan, P.;Storeng, S.;Studiero, D.;Monke, A.;McMahon, J.;Vistica, D.;Warren, J.;Bodesh, H.;Kenny, S.;Boyd, M.R.