Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Cytotoxicity of the Methanol Extract of Crotalariae sessiliflorae L.

농길리 메탄올 추출물의 세포독성

  • Han Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Chung Woo-Young (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Park Myung-Oh (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Shin Min-Kyo (Department of Herbalogy, School of Oriental Medicine, Wonkwang University) ;
  • Oh Hyun-Ju (Department of Herbal Resources Development, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Baek Seung-Hwa (Department of Herbal Resources Development, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 정우영 (원광대학교 치과대학 구강해부학교실) ;
  • 박명오 (원광대학교 치과대학 구강해부학교실) ;
  • 신민교 (원광대학교 한의과대학 본초학교실) ;
  • 오현주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2001.06.29
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Abstract

The cytotoxic activity of Cratalariae sessiliflorae on cultured NIH 3T3 fibroblasts and human oral epithelioid carcinoma cells (KB) were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) colorimetric method These fractions of methanol extract of Cratalariae sessiliflorae showed inhibitory effect in vitro in the milligram range against KB cell lines. In general, the antitumor activities of these fractions were does-dependent over the milligram range. The comparison of IC50 values of these fractions in tumor cell lines showed that their susceptibility to these fractions decrease in the following order: Fr. 4> Fr. 6> Fr. 10> Fr. 2> Fr. 11> Fr. 3> Fr. 8> Fr. 7> Fr. 9> Fr. 1> Fr. 5 by the MTT assay. These fractions were tested for their cytotoxic effects on NIH 3T3 fibroblasts using MTT assay. They exhibited potent cytotoxic activities in vitro in the milligram range against NIH 3T3 fibroblasts. In general, the cytotoxic activities of these fractions were does-dependent over the milligram range. The comparison of CD50 values of these fractions in NIH 313 fibroblasts shows that their susceptibility to these fractions in decrease the following order: Fr. 10> Fr. 9> Fr. 2 = Fr. 4> Fr. 8> Fr. 11> Fr. 1 = Fr. 7> Fr. 3> Fr. 5 = Fr. 6 by the MTT assay. These results suggests that fraction 5 has the most growth - inhibitory activity against KB cell lines.

Keywords

References

  1. Goodman Gillman's: The Pharmacological Basis of Thrapeuties Gillman, A.G.;Rall, T.W.;Nies, A.;Talyor, P.
  2. Methods in Cancer Research Hersh, E.M.;Ereish, E.T.
  3. Biosci. Biotech. Biochem. v.56 Priming effects of vegetable Juice on endogeneous products of tumor nectrosis factor Yamazaki, M.;Ueda, H.;Fukda, K.;Okamoto, M.;Yui, S.
  4. Chem. Pharm. Bull. v.35 Effects of adriamycin cultured mouse embryo mycardial cells Takahashi, K.;Fujita, Y.;Mayumi, T.;Hanma, T.;Kishi, T.
  5. Exp. Cell. Res. v.41 Effects of inhibitors of protein synthesis on the development of preimplantation mouse embryos Tompson, J.;Biggers, J.D.
  6. Exp. Cell. Res v.71 Differential effects of metabolic inhibitors on early development in the mouse embryos at various stage of the cell cycle Molinaro, M.;Siracusa, G.;Monesi, V.
  7. Devel. Biol. v.118 Patters of nucleic acid synthesis in the early mouse embryos Ellem, K.O.;Gwatkin, R.B.L.
  8. Proc. Cell Natur. Sci. v.1 Effects of progesterone and dibutyryl cyclic AMP on the RNA and protein synthesis of mouse oocytes and two cell embryos in vitro Cho, K.W.;Kwon, H.B.
  9. Recent Advances in the Pharmacology of Kampo Medicine Aburada, M.
  10. Recent Advances in the Pharmacology of Kampo Medicine Kawamura, H.;Takemoto, N.;Maruyama, H.;Komatsu, Y.;Aburada, M.;Hosoya, E.
  11. Recent Advances in the Pharmacology of Kampo Medicine Takemoto, N.;Maruyama, H.;Kawamura, H.;Komatsu, Y.;Aburada, M.;Hosoya, E.;Yamada, H.;Klyohara, H.;Cyong, J.C.
  12. 대한구강 해부학회지 v.18 소엽의 메탄올 분획이 피부암세포에 미치는 항암효과 한두석;유현경;백승화
  13. Kor. J. Pharmacogn. v.30 Development of anticancer agents from Korean medicinal plants. Part 13. Studies on the cytotoxicity and antitumor activity of Herba Crotalariae sessiliflorae Shin, M.K.;Song, M.J.;Ryu, H.S.;Han, D.S.;Kang, K.U.;Baek, S.H.
  14. 임상본초학 신민교
  15. 도해향약 (도해) 대사전 정보섭;신민교
  16. 중국약물대전 중국약물대전편위회(編)
  17. J. Immunol. Methods v.65 Rapid colorimetric assays for cellular growth and survival; Aplication of proliferation and cytotoxicity assays Mosmann, T.