Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Effects of Gamisoyosan(GS) on LDL Oxidation in RAW 264.7 Cell.

가미소요산(加味逍遙散)이 지단백산화(脂蛋白酸化)에 따른 RAW 264.7 활성(活性)에 미치는 영향(影響)

  • Hwang Gwi-Seo (College of Oriental Medicine, Kyungwon University)
  • 황귀서 (경원대학교 한의과대학)
  • Published : 2001.06.29
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Abstract

The oxidative modification of low density lipoprotein(LDL) has been implicated in the development of atherosclerosis. Oxidized LDL are found in macrophage foam cell, and it can induce an macrophage proliferation in atherosclerotic plaque. In this study, we investigated the hypothesis that gamisoyosan(GS) may reduce atherosclerosis by lowering the oxidiazability of LDL, To achive this goal, we examined the effect of GS on LDL oxidation, nitric oxide production in mouse macrophage cell line, RAW264.7, and the effect of GS on cupuric sulfate-induced cytotoxicity, LDH release, and macrophage activity. GS inhibited the generation of oxidized LDL from native LDL in RAW264.7 cell culture, and decreased the release of LDH from cupric sulfate-stimulated RAW264.7 cell. In other experiments, GS activated RAW264.7 cell, and prolonged the survival time, and increased nitric oxide production in Raw 264.7 cells.

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References

  1. JAMA v.264 Lipoproteins and atherosclerosis, Current concepts Steinberg, D.;Witztum, J.L.
  2. Mayo Clin Proc v.75 Novel risk factors for atherosclerosis Kullo, K.J.;Gau, G.T.;Tajik, J.
  3. Am J Cardiol v.85 Correlation of homocysteine levels with the extent of coronary atherosclerosis in patients with low cardiovascular risk profiles Tsai, W.C.;Li, Y.H.;Tsai, L.M.;Chao, T.H.;Lin, L.J.;Chen, T.Y.;Chen, J.H.
  4. Curr Opin Lipidol v.11 Macrophage proliferation in atherosclerosis Sakai, M.;Kobori, S.;Miyazaki, A.;Horiuchi, S.
  5. Atheriosclerosis v.10 Macrophage and smooth muscle proliferation in atherosclerotic lesions of WHHL and comparably hypercholesterolemic fat-fed rabbits Rosenfeld, M.E.;Ross, R.
  6. J Biol Chem v.271 Interleukin-8 is induced by cholesterol loading of macrophage and expressed by macrophage foam cells in human atheropma Wang, N.;Tabas, I.;Wichester, R.;Ravalli, S.;Rabbani, L.E.;Tall, A.
  7. Mol Cell Biol v.10 Activation of granulocyte-macrophage colony stimulating factor promotor in T cells requires cooperative binding of Elf-1 and AP-1 transcription factors Wagn, C.Y.;Bassul, A.G.;Boise, L.H.
  8. J Clin Invest v.88 Role of oxidized low density lipoprotein in atherosclerosis Witztum, J.L.;Steinberg, D.
  9. Blood v.71 Granulocyte/macrophage colony stimulating factor stimulates monocyte and tissue mactophage proliferation and enhances their responsiveness to macrophage colony stimulating factor Chen, B.M.;Clark, C.R.;Chou, D.L.
  10. Biochemistry v.35 Oxidized low density lipoprotein stimulates protein kinase C (PKC) and induces expression of PKC isotypes via prostaglandin H synthetase in P288D1 macrophage-like cells Claus, R.;Fyrnys, B.;Deigner, H.P.;Wolf, G.
  11. 東醫方劑와 處方解說 尹甲用
  12. Kor. J. Environ. Toxicol. v.13 Effect of Danchisoyosan on catecholamine level in rat brain applied to immobilization stress Hwang, G.S.;Lee, K.S.;Park, J.H.
  13. Throm Res v.47 Endothelium derived relaxing factor from cultured human endothelial cell inhibits aggregation of human platelets Alheid, U.;Frolich, J.C.;Forstermann, U.
  14. Clin Exp Immunol v.87 Inhibition of the reactive proliferation of lymphocytes by activated macrophages: the role of nitric oxide Denham, S.;Rowland, I.J.
  15. Agents Actions v.45 Activation of endothelial guanylate cyclase inhibits cellular reactivity Heller, R.;Bussolino, F.;Ghigo, D.;Pescarmona, G.P.;Calvino, R.;Gasco, A.;Till, U.;Bosia, A.
  16. Biochem Biophys Res Commun v.171 Nitric oxide decreases cytosolic free calcium in Balb/c 3T3 fibroblasts by cyclic GMP-dependent mechanism Garg, U.C.;Hassid, A.
  17. J Clin Invest v.99 3-Chlorotyrosine, a specific marker of myeloperoxidase-catalysed oxidation, is markedly elevated in low density lipoprotein isolated from human atherosclerotic intima Hazen, S.L.;Heinecke, J.W.
  18. J Biol Chem v.272 Mass spectrometric quantitation of marker for protein oxidation by tyrosyl radical in low density lipoprotein isolated from human atherosclerotic plaques Leuwenburg, C.;Rasmussrn, J.E.;Hsu, F.F.
  19. J Biol Chem v.271 Molecular chlorine generated by the myeloperoxidase-hydrogen peroxide- chlorine system of phagocytes converts low density lipoprotein cholesterol into a family of chlorinated sterols Hazen, S.L.;Hsu, F.F.;Duffin, K.;Heinecke, J.W.