Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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The Studies on Search of Antifibrotic Effect by Several Herbs Extract in Rat Liver Tissue

수종(數種)의 한약재(韓藥材) 전탕액(煎湯液)이 동물 간조직내 항섬유효능검색에 관한 실험적 연구

  • Seo Dong-Jin (Dept. of Preventive Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Chun-Woo (Dept. of Preventive Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Hong-Gi (Dept. of Preventive Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Ki-Nam (Dept. of Preventive Medicine, College of Oriental Medicine, Wonkwang University)
  • 서동진 (원광대학교 한의과대학 예방의학교실) ;
  • 이춘우 (원광대학교 한의과대학 예방의학교실) ;
  • 김홍기 (원광대학교 한의과대학 예방의학교실) ;
  • 이기남 (원광대학교 한의과대학 예방의학교실)
  • Published : 2001.06.29
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Abstract

Antifibrotic drugs could be delayed or inhibited massive collagen deposition in liver tissue or inhibited collagen synthesis. we investigated antifibrotic effects by several herbs(Schisnadra chinensis, Ganoderma japonicum, Sedum sarmentosum, Alisma canaliculatum, Plantago asiatica) extract with observations of collagen accumulation in liver tissue and collagen synthesis in sera. Rats were used for experimental animal that were devided 3 groups(sham control, BDL/S, BDL/S-ER). Rats were operated for inducing liver fibrosis(cirrhosis) by bile duct obstruction. Several herbs were prepared by water extraction and were applicated p. o. $3ml/day$ during 4 weeks. After observation period, rats were sacrificed and liver tissue and sera were collected. In result, the mortality of rats was 35% in BDL/S group and 20% in BDL/S-ER The color of bile juice in BDL/S-ER was bright yellow and murky yellow in BDL/S group. The significantly lower weight of liver($16.21g{\pm}5.3,\;20.58{\pm}2.4$) and spleen($1.96g{\pm}0.96,\;3.93{\pm}0.21$) were shown in BDL/S-ER than that of BDL/S(p<0.05) group. The value of collagen in liver tissue(25.7%) in BDL/S-ER was observed significantly lower than that of BDL/S group (Tab. 2). AST. ALT, ALP, t-bilirubin, BUN levels were low in BDL/S-ER as compared with those of BDL/S group, but the significance was not proven. The trichrome stained liver tissue in BDL/S-ER group was observed mild bile duct proliferation and fibrosis compared with BDL/S group. In conclusion, natural products inhibited new collagen synthesis and delayed massive collagen deposition in liver tissue, so that they have noticeable antifibrotic effects in experimental liver fibrosis(cirrhosis).

Keywords

References

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