The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Sagunjatang and Sagunja-tang plus Mylabris phalerata on Human Stomach Cancer Cells

사군자탕(四君子湯) 및 사군자탕가반묘(四君子湯加斑猫)가 위암세포에 미치는 영향

  • Jung, Woo-Young (Department of 3rd Internal Medicine, Oriental Medicine, Kyung Hee University) ;
  • Ryu, Bong-Ha (Department of 3rd Internal Medicine, Oriental Medicine, Kyung Hee University) ;
  • Kim, Jin-Sung (Department of 3rd Internal Medicine, Oriental Medicine, Kyung Hee University) ;
  • Yoon, Sang-Hyub (Department of 3rd Internal Medicine, Oriental Medicine, Kyung Hee University) ;
  • Ryu, Ki-Won (Department of 3rd Internal Medicine, Oriental Medicine, Kyung Hee University) ;
  • Sin, Hyeon-Su
  • 정우영 (경희대학교 한의과대학 비계내과학교실) ;
  • 류봉하 (경희대학교 한의과대학 비계내과학교실) ;
  • 김진성 (경희대학교 한의과대학 비계내과학교실) ;
  • 윤상협 (경희대학교 한의과대학 비계내과학교실) ;
  • 류기원 (경희대학교 한의과대학 비계내과학교실) ;
  • 신현수 (동서한방병원)
  • Published : 2001.12.30
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Abstract

The efficacy of Sagunja-tang and Sagunja-tang plus Mylabris phalerata against the human stomach cancer was examined and molecular biological fight of its actions was studied. In the efficacy test of anti-stomach cancer cells growth using the MTT assay, administration of Sagunja-tang resulted in no significant change of stomach cancer cells growth, with the control group. Administration of Sagunja-tang plus Mylabris phalerata resulted in a decrease of stomach cancer cells growth in proportion to the concentration of mylabris phalerata and time, which was significantly different from the control group(significance recognized when p<0.05). In the test using the apoptosis assay, administration of Sagunja-tang showed an increase in apoptosis of human stomach cancer cells, with no significant difference from the control group. Administrating Sagunja-tang plus Mylabris phalerata showed an increase in apoptosis of stomach cancer cells in proportion to the concentration of mylabris phalerata and time, which was significantly different from the control group(significance recognized when p<0.05). In the test using the quantitative RT-PCR to examine stomach cancer cells growth and revelation of apoptosis related genes, administrating Sagunja-tang plus Mylabris phalerata resulted in a decrease of Bcl-2, an anti-apoptosis gene, in proportiong to concentration. No significant change was examined in the revelation of CDK1, Cdc2, Cyclin D1, PCNA, c-myc, which are genes related to the stomach cancer cells growth, and Bax, Bel-XL, the genes related to apoptosis, and p53. Referring to the results above, Sagunja-tang plus Mylabris phalerata may be considered to have an anti-growth efficacy against human stomach cancer cells, and an inducement efficacy. Therefore, it can be clinically implemented in the human stomach cancer.

Keywords

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