The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effects of Jeongcheon-tang and Cheongsangboha-tang on IL-4, IL-5, Il-6 and IL-10 in Asthma Model

정천탕(定喘湯)과 청상보하탕(淸上補下湯)이 asthma model 내(內)의 cytokine에 미치는 영향(影響)

  • Jung, Hee-Jae (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Rhee, Hyung-Koo (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Jung, Sung-Ki (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Young-Woo (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
  • 정희재 (경희대학교 한의과대학 폐계내과학교실) ;
  • 이형구 (경희대학교 한의과대학 폐계내과학교실) ;
  • 정승기 (경희대학교 한의과대학 폐계내과학교실) ;
  • 김영우 (경희대학교 한의과대학 폐계내과학교실)
  • Published : 2001.09.30
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Abstract

Objectives: We aimed to identify. the effect of Jeongcheon-tang(定喘湯) and Cheongsangboha-tang(淸上補下湯) on the transcriptional activities of cytokine IL-4, IL-5, IL-6 and IL-10 involved in asthma model. Materials and Methods: RBL-2H3 cell lines were used. Cells were stimulated with calcium inophore($2{\mu}M$ : Sample group 1, $4{\mu}M$ : Sample group 2) for maximal gene expression. After 3rd treatment of samples and incubation(per 24hours), total cellular RNAs were collected using Trizol solution method. Then transcriptional activities of IL-4, IL-5, IL-6 and IL-10 were measured by RT-PCR with electrophoresis. Results: In IL-4 study, Jeongcheon-tang treated group showed 82.76%(Sample group 1) of transcriptional activities compared to the control group and Cheongsangboha-tang treated groups showed 85.77% (Sample group 1), 89.42% (Sample group 2) of transcriptional activities compared to the control groups. In IL-5 study, Jeongcheon-tang treated groups showed 88.24%(Sample group 1), 98.83%(Sample group 2) of transcriptional activities compared to the control groups and Cheongsangboha-tang treated group showed 73.66%(Sample group 2) of transcriptional activities compared to the control group. In IL-6 study, Jeongcheon-tang treated group showed 92.95%(Sample group 2) of transcriptional activities compared to the control group and Cheongsangboha-tang treated group showed n.40%(Sample group 2) of transcriptional activities compared to the control group. In IL-10 study, Jeongcheon-tang treated group showed 118.46% (Sample group 2) of transcriptional activities compared to the control group. Conclusions: This study shows that Jeongcheon-tang has the inhibitory effect on the transcription of IL-4, IL-5, IL-6 gene expression and the increasing effect on the transcription of IL-10 gene expression, and Cheongsangboha-tang has the inhibitory effect on the transcription of IL4, IL-5 and IL-6 gene expression in RBL-2H3 cell lines. Advanced studies are required to investigate the mechanisms of inhibition or increase by herbal medicine in asthma model.

Keywords

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