대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

우황청심원(牛黃淸心元)이 저산소증 유발 배양신경세포에 미치는 영향

The Effects of Woohwangcheongsim-won on Hypoxic E18 Cortical Neuroblast

  • 조규선 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실) ;
  • 문일수 (동국대학교 의과대학 해부학교실) ;
  • 이원철 (동국대학교 한의과대학 내과학교실) ;
  • 정승현 (동국대학교 한의과대학 내과학교실)
  • Cho, Gyu-Seon (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Shin, Gil-Cho (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Moon, Il-Soo (Department of Anatomy, College of Medicine, Dongguk University) ;
  • Lee, Won-Chul (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Chung, Sung-Hyun (Department of Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 발행 : 2001.06.30
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초록

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast. Methods : To evaluate the effect of Woohwangcheongsim-won on neuronal death caused by hypoxia, the survival rate of E18 cortical neuroblast was measured with MTT assay and the changes of several synaptic proteins and enzymes were investigated with the immunoblot assays. Results : The E18 cortical neuroblasts were added 50, 100, 500, 1,000, and $5,000{\mu}g/ml$ Woohwangcheongsim-won. They showed neurotoxicity, when the concentration of Woohwangcheongsim-won was above $1,000{\mu}g/ml$. The E18 cortical neuroblasts, which were added 50, 100, and $500{\mu}g/ml$ Woohwangcheongsim-won, were exposed 98% $N_2/5%\;CO_2$ for 3 hours to induce hypoxia, 3 days later, the survival rate of $50{\mu}g/ml$ Woohwangcheongsim-won was 141.5% when compared to the control group. On the immuneblot assays, the expressions of ${\alpha}$CaMKII, NR2A, NR28, PDE2, PSD-95, and eEF-$1{\alpha}$ were increased in normoxia, but those of NR2A, NR2B were decreased in hypoxia when compared to the control group. Conclusions : The data shows that the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast is a significant result.

키워드

참고문헌

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