대한한방종양학회지 (THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY)

대한암한의학회 (Korean Association of Traditional Oncology)
권호 표지

목향(木香)과 차전초(車前草)가 위암세포(胃癌細胞)의 활성(活性), 증식(增殖), 자기살해능(自己殺害能) 및 세포주기관련 유전자 발현에 미치는 영향

The Effects of Saussurea Radix and Plantaginis Herba on Cellular Viability, Proliferation, Apoptosis and Expression of Cell Cycle-related Genes in Gastric Cancer Cells

  • Oh, Hee-Rah (Department of Internal Medicine, College of Oriental Medicine, Sangji University) ;
  • Ko, Seong-Gyu (Department of Internal Medicine, College of Oriental Medicine, Sangji University)
  • 발행 : 2001.12.30
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초록

Objective: This experimental study was carried out to evaluate the effects of Saussurea Radix and Plantaginis Herba on cellular viability, proliferation, apoptosis and expression of the cell cycle-related genes in cultured gastric cancer cells. Method :MTT assay for analysis of cellular toxicity and the effect on suppression of cellular viability, $[^{3}H]$ thymidine incorporation assay for evaluation of the effect on suppression of DNA replication, tryphan blue exclusion assay for measurement of induction of apoptosis and Quantitative RT-PCR for analysis of the effects on expression of cell cycle or apoptosis-related genes were performed. Results: Antitumor activity of Saussurea Radix associated with inhibition of cell cycle progression and promotion of apoptosis caused by transcriptional regulation of p53, p21/Wafl and the other related genes was observed.

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참고문헌

  1. 대한한의학회지 v.19 no.1 韓醫學界의 癌硏究動向과 硏究 戰略에 대한 硏究 金聖勳
  2. 종양학 서울대학교 의과대학
  3. Cell v.74 Molecular regulation of apoptosis : genetic controls on cell death. Williams, G.T.;Smith, C.A.
  4. 癌細胞 增殖抑制에 대한 木香 및 良薑 추출물의 영향연구 李瑞衡
  5. 차전초 수침액이 실험적 당뇨와 간기능 손상에 미치는 영향 김정인
  6. 질경이, 씀바귀 및 뽕잎의 열수 추출액이 흰쥐의 간 손상 회복에 미치는 영향 백경연
  7. Cancer chemother Pharmacology v.17 no.3 Rapid chemosensitivity testing of human lung tumor cells using the MTT assay Cole, S.P.
  8. Nature v.352 Wild-type p53 induces apoptosis of myeloid leukemic cells that is inhibited by interleukin-6. Yonish-Rouach, E.;Resnitzky, D.;Lotem,J.;Sachs, L.;kimchi, A.;Oren, M.
  9. J. cell. Biochem. v.54 control of fibroblast senescence and activation of programmed cell death. Wang, E.;Lee, M.J.;Pandey, S.
  10. 대한한방종양학회지 v.2 no.1 積聚 處方에 대한 文獻的考察 문구;조성각
  11. 대전대학교 한의과 대학 논문집 v.3 no.2 數種 韓藥材의 抗癌活性 硏究 康坼林
  12. 완역중약대사전 김창민;신민교;이경순;안덕균(외)
  13. 대전대학교 한의과 대학 논문집 v.4 no.1 인체 위암세포주 SNU-1에 대한 수종 한약재의 항암활성 康坼林
  14. 한국한의학연구원 보고서 수종한약재의 위암세포에 대한 항암작용 효능검색 및 약리작용에 관한 분자생물학적 연구
  15. Journal of immunologic methods v.65 no.1-2 Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays Mosmann, T.
  16. Br. J. Cancer v.67 apoptosis Wyllie, A.H.
  17. Eur Respir J, v.9 no.6 The role of apoptosis in the pathogenesis and treatment of diseases. Solary, E.;Dubrez, L.;Eymin, B.
  18. Biol Rev Camb Philos Soc v.67 no.3 Programmed cell death:concept, mechanism and control. Sen, S.
  19. Genes Dev. v.6 Wild-type p53mediates positive regulation of gene expression through a specific DNA sequence element Zambetti, G.;Bargonetti, J.;Walker, K.;Prives, C.;Levine, A.J.
  20. Proc. Natl. Acad. Sci. v.88 The MCK enhancer contains a p53 responsive element. Weintraub, H.;Hauschka, S.;Tapscott, S.J.
  21. EM8O v.12 Mdm-2 expression is induced by wild-type p53 activity. Barak, Y.;Juven, T,;Haffner, R.;Oren, M.
  22. Science v.256 Oncogenic forms of p53 inhibit p53-regulated gene expression. Kern, S.E.;Pietenpol, J.A.;Thiagalingam, S.;Seymour, A.;Kinzler, K.W.;Vogelstein, B.
  23. Cell v.71 A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. Kastan, M.B.;Zhan, Q.;El-Deiry, W.S.;Carrier, F.;Jacks, T.;Walsh, W.V.;Plunkett, B.S.;Fornace, A.J.Jr.
  24. Proc. Natl. Acad. Sci. v.88 Wild-type p53 can down-modulate the activity of various promoters. Ginsberg, D.;Mechta, F.;Yaniv, M.;Oren, M.
  25. Nature v.363 Specific repression of TATA-medlated but not initiator-mediated transcription by wild-type p53. Mack, D.H.;Vartikar, J.;Pipas, J.M.;Laimins, L.A.
  26. Proc. Natl. Acad. Sci. v.89 Wild-type p53 binds to the TATA-binding protein and represses transcription. Seto, E.;Usheva, A.;Zambetti, C.P.;Momand, J.;Horikoshi, N.;Weinmann, R.;Levine, A.J.;Shenk, T.
  27. J. Biol. Chem. v.268 Induction of Spl-p53 binding heterocomplexes during granulocy to macrophag e colony-stimulating factor-dependent proliferation in human orythreleukernia cell line TF-1 Borellini, f.;Glazer, R.I.
  28. Science v.255 Modulation of activity of the promoter of the human MDRI gene by ras and p53. Chin, K.V.;Ueda, K.;Pastan, I.;Gottesman, M.M.
  29. J. Bioenerg. Biomembr. v.22 Genetic basis of multidrug resistance of tumor cells. Kane, S.E.;Pastan, I.;Gottesman, M.M.
  30. Proc. Natl. Acad. Sci. v.81 Negative growth regulation in a glioblastoma tumor cell line that conditionally expresses human wild-type p53. Mercer, W.E.;Shields, M.T.;Amin, M.;Sauve, G.J.;Appella, E.;Romano, J.W.;Ullrich, S.J.
  31. Proc. Natl. Acad. Sci. v.91 Cdk-interacting protein 1 directly binds with proliferating cell nuclear antigen and inhibits DNA replication catalyzed by the DNA polymerase holoenzyme. Flores-Rozas, H.;Kelman, Z.;Dean, F.B.;Pan, Z.Q.;Harper, J.W.;Elledge, S.J.;0'donnell, M.;Hurwitz, J.
  32. Proc. Natl. Acad. Sci. v.88 Growth suppression induced by wild-type p53 protein is accompanied by selective down-regulation of proliferating-cell nuclear antigen expression. Mercer, W.E.;Shields, M.T.;Lin, D.;Appella, E.;Ullrich, S.J.
  33. Cancer Res. v.54 Wafl/CIP1 is induced in p53-mediated G1 arrest and apoptosis. El-Deiry, W.S.;Harper, J.W.;0'Conner, P.M.;Velculescu, V.E.;Canman, C.E.;Jackman, J.;Pietenpol, J.A.;Brrrell, M.;Hill, D.E.;Wang, Y.;Wiman, K.G.;Mercer, W.E.;Kastan, M.B.;Kohn, K.W.;Elledge, S.J.;Kinzler, K.W.;Vogelstein, B.
  34. Cell v.75 The p21 cdk-Interacting protein Cipl is a poten tinhibitor of G1 cyclin-dependent kinases Harper, J.W.;Adami, G.R.;Wel, N.;Keyomarsi, K.;Elledge, S.J.
  35. Cell v.75 Wafl, a potential mediator of p53 tumor suppression. El-Deiry, W.S.;Tokino, T.;Velculescu, V.E.;Levy, D. B.;Parsons, R.;Trent, J.M.;Lin, D.;Mercer, W.E.;Kinzler, K.W.;Vogelstein, B.
  36. Proc .Natl. Acad. Sci. v.92 Exit from GO and entry into the cell cycle of cells expressing p2lSdi1 antisense RNA. Nakanishi, M.;Adami, G.R.;Robetorye, R.S.;Noda, A.;Venable, S.F.;Dimitrov, D.;Pereira-Smith, 0.M.;Smith, J.R.
  37. the journal of immunology v.151 no.2 Effect of bcl-2 on Fas antigen-mediated cell death Itoh, N.;Tsujimoto. Y..Nagata, S.
  38. proc. natl. Acad. Sci. v.94 no.21 Comparison of the ionchannol characteristics of proapoptotic BAX and antiapoptotic BCL-2. Schlesinger, P.H.;Gross, A.;Yin, A.M.;Yamanoto, K.;Saito, M.;Waksman, G.;Korsmeyer, S.J.
  39. Blood v.88 no.5 Upregulated Expression of Bcl-2 in multiple myeloma cells induced by exposure to Doxorubicin, Etoposide, and Hydrogenperoxide, Tu, Y.;Feng-hao Xu;Liu, Jin;Versco, R.;Berenson, J.;Fady, C.;Lichtanstein, A.
  40. Blood v.88 no.10 Flt3 ligand stimulates proliferation and inhibits apoptosis of acute myeloid leukemiacells:Regulation of Bcl-2 and Bax, Lisovsky, M.;Estrov, Z.;Zhang, X.;Consoli, U.;Sanchez-Williams, G.;Snell, V.;Munker, R.;Goodacre, A.;Savchenko, V.;Andreeff, M.
  41. Blood v.89 no.8 Expression and regulation of Bcl-2, Bcl-xL, Bax correlate with p53 status and sensitivity to apoptosis in childhood acute Iymphoblastic leukemia. Findly, H.W.;Gu, L.;Yeager, A.M.;Zhou, M.
  42. Proc. Natl. Acad. Sci v.93 BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway Strobel, T.;Swanson, L.;Korsmeter, S.;Cannistra, S.A.
  43. nature v.385 Bax suppresses tumorigenesis and stimulates apoptosis in vivo. Yin, C.;Knudson, C.M.;Korsmeyer, S.J.;Van-Dyke, T.
  44. PCR Methods and Applications v.2 Quantitative or semi-Quantitative PCR:reality versus myth. Ferre, F.
  45. Proc. Natl. Acad. Sci. v.86 no.24 Quantitation of mRNA by Polymerase chain reaction. Wang, A.M.W.;Doyle, M.V.;Mark, D.F.
  46. 대한한방종양학회지 v.1 no.1 癌腫의 病因病理에 대한文獻的 考察 全炳旭;柳逢夏;朴東源;柳基遠
  47. 原色 臨床本草學 辛民敎