Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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The Effects of Sagunja-tang on Liver Injury of Mice Induced by $CCl_4$

사군자탕(四君子湯)이 $CCl_4$에 의한 생쥐의 간 조직 손상에 미치는 영향

  • 김영균 (동의대학교 한의과대학 내과학교실) ;
  • 조수인 (동신대학교 한의과대학 본초학교실)
  • Published : 2001.12.20
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Abstract

Objectives : This study was carried out to research the protective effects of Sagunja-Tang(SA) through in vivo experiments, and tried to investigate the relation between oxidation of liver tissues and deficiency of Qi. Methods : Acute liver injury which initiated from free radical induced by $CCl_4$, were applied to mice and metabolic data were obtained. In order to measure the degree of liver injury, serum level of alanine aminotransferase(AST), aspartate aminotransferase(ALT), creatinine, blood urea nitrogen(BUN), total protein(TP) and glucose were measured. Lipid peroxidation of liver slice was examined by measuring malondialdehyde(MDA), a product of lipid peroxidation. Results : SA had protective effects on $CCl_4$ induced acute liver injury by decreasing serum level of ALT. Kidney injury was induced by injection of $CCl_4$ too, and SA protected kidney injury by decreasing serum level of creatinine and BUN. Conclusions : Through this study, we found that SA have healing effects on liver and kidney injury of $CCl_4$ induced oxidative stress that is similar to deficiency of Qi. And further studies have to be followed to certify the mechanisms.

Keywords

References

  1. 太平惠民和劑局方 陳師文(等編)
  2. 天眞處方解說 成輔社(編譯)
  3. 精校黃帝內經素問 洪元植(校合編纂)
  4. 精校黃帝內經靈樞 洪元植(校合編纂)
  5. 신물질탐색 신물질탐색연구회(편저)
  6. J. Pharmacol. Exp. Ther. v.258 no.2 U-78517F: A potent inhibitor of lipid peroxidation with activity in experimental brain injury and ischemia Hall E. D.;Braughler J. M.;Yonkers P. A.;Smith S. L.;Linseman K. L.;Means E. D.;Scherch H. M.;Von Voigtlander P. F.;Lahti R. A.;Jacobsen E. J.
  7. Anal. Biochem Vol. v.86 Determination of malonaldehyde precusor in tissue by thiobarbituric acid test. Uchiyama M.;Mihara M.
  8. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dry binding. Bradford M. M.
  9. 虛證論 索延昌
  10. 中藥名方藥理與應用 珍奇
  11. J. Amer. Geriatr. Soc. v.20 The Biologic clock: the mitochondria? Harman D.
  12. Free Rad. Res. v.21 Low mitochondrial free radical production per unit O2 consumption can explain the simultaneous presence of high longevity and high aerobic metabolic rate in birds Barja G.;Cadenas S.;Rojas C.(et al.)
  13. Science v.273 Oxidative stress, caloric restriction, and aging Sohal R. S.;Weindruch R.
  14. Free Rad. Biol. Med. v.21 Aging and oxidative stress : modulation by dietary restriction Yu B. P.
  15. Biochemical Pharmacology v.52 The Crucial Antioxidant Action of Schisandrin B in Protecting Against Carbon Tetrachloride Hepatotoxicity in Mice : A Comparative Study with Butylated Hydroxytoluene Ip S. P.;Ko K. M.
  16. Biological & Pharmaceutical Bulletin. v.20 no.4 Hepatoprotective Effect of Hovenia Dulcis THUNB. on Experimental Liver Injuries Induced by Carbon Tetrachloride or D-Galactosamine/Lipopolysaccharide Hase K.;Ohsugi M.;Xiong Q. B.
  17. Toxicology & Applied Pharmacology v.143 Uptake, Distribution, and Elimination of Carbon Tetrachloride in Rat Tissues Following Inhalation and Ingestion Exposures Sanzgiri U. Y.;Srivatsan V.;Muralidhara S.
  18. Toxicology & Applied Pharmacology v.148 Evaluation of Urinary Biomarkers for Radical-Induced Liver Damage in Rats Treated with Carbon Tetrachloride Dezwart L. L.;Hermanns R. C. A.;Meerman J. H. N.
  19. Hepatology v.28 no.6 Interleukin-10 controls neutrophilic infiltration, hepatocyte proliferation, and liver fibrosis induced by carbon tetrachloride in mice Louis H.;Van Laethem J. L.;Wu W.(et al.)
  20. Biological &Pharmaceutical Bulletin v.21 no.10 Effects of dietary unsaturated fatty acid and chronic carbon tetrachloride treatment on the accumulation of oxidation products, alpha-tocopherol and liver injury in mice Yasuda S.;Watanabe S.;Kobayashi T.