Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)
- Volume 28 Issue 4
- /
- Pages.709-727
- /
- 2001
- /
- 1226-8496(pISSN)
- /
- 2288-3819(eISSN)
TEMPOROSPATIAL PATTERNS OF PROGRAMMED CELL DEATH DURING EARLY DEVELOPMENT OF THE MOUSE EMBRYOS
생쥐 배자발생초기의 세포자기사 발현 양상에 관한 연구
- Baik, Byeong-Ju (Department of Pediatric Dentistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
- Lee, Seung-Ik (Department of Pediatric Dentistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
-
Kim, Jae-Gon
(Department of Pediatric Dentistry, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
- Park, Byung-Yong (Department of oral Anatomy and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
- Park, Byung-Keon (Department of oral Anatomy and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
- 백병주 (전북대학교 치과대학 소아치과학교실, 구강생체과학연구소) ;
- 이승익 (전북대학교 치과대학 소아치과학교실, 구강생체과학연구소) ;
-
김재곤
(전북대학교 치과대학 소아치과학교실, 구강생체과학연구소) ;
- 박병용 (전북대학교 치과대학 구강해부학교실, 구강생체과학연구소) ;
- 박병건 (전북대학교 치과대학 구강해부학교실, 구강생체과학연구소)
- Published : 2001.11.30
Abstract
The pattern of programmed cell death(PCD) has been examined during the early developmental period of development in mouse embryos, from embryonic day 4.5(E4.5) to E11.5 Embryos from Balb/c breedings were harvested at various embryonic stages between E4.5 and El1.5. Cell death was analysed by in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL) staining in tissue sections and whole embryos. At the blastocyst stage(E4.5), a very few apoptotic cells were found in the inner cell mass of the blastocyst. In the early egg cylinder stage(35.0-5.5), a few apoptotic cells were detected in the embryonic ectoderm, the embryonic endoerm and the proamniotic cavity. In the advanced egg cylinder stage(E5.5-6.5), TUNEL-posifive cells were observed in the extra-embryonic ectoderm and extra-embryonic endoderm as well as in the embryonic ectoderm, embryonic visceral endoderm and proamniotic cavity. In the streak stage(E6.75-7.75), many TUNEL-positive cells were found in the ectoplacental cone. In contrast, only very few apoptotic cells were found in the chorion and extra-embryonic endoderm in extra-embryonic regions. In intra-embryonic region, a few apoptotic cells were randomly found in the embryonic ectoderm, mesoderm and visceral endoderm. At the early somitogenesis stage(E8.0-8.5), most apoptotic cells were observed in the most cranial portion of neural fold (neural ectoderm and adjacent ectoderm). At the mid somitogenesis stage(39.0-9.5), the otic placode first showed TUNEL-positive at this stage. Small number of TUNEL-positive cells were also first seen around optic placode and branchial arches. Three streams of TUNEL-positive cells were clearly seen in the cranial region at 59.5-9.75. At E10.5, apoptotic cells were localized in the developing eye, the junctional portion of medial nasal, lateral nasal and maxillary processes, the lateral portion of branchial arches, the junction of bilateral mandibular processes, and apical ectodermal ridges of limb buds. At E11.5, apoptotic cells were noticeably decreased in most area, except the developing limbs and several somites in the tail region. In this study, the global temporospatial pattern of PCD throughout early development of mouse embryos was discussed. It may provide the basis for further studies on its role in the morphogenesis of the embryo.
세포자기사(programmed cell death)는 배자발생과정에서 중요한 역할을 하는 정상적인 생리적현상으로 알려져왔다. 생쥐 배자에서 세포자기사에 관한 많은 연구가 있었지만 초기 형태발생기동안의 전반적이고 구체적인 세포자기사 양상에 관한 보고는 없었다. 이에 본 연구에서는 발생 4.5일