대한추나의학회지 (The Journal of Korea CHUNA Manual Medicine)

척추신경추나의학회 (Korean Society of Chuna manual Medicine for Spine and Nerves)
권호 표지

백굴채(白屈菜)가 손상된 배양척수감각신경세포에 미치는 영향

Effects of Herbar Chelidonii on the Cultured Spinal Sensory Neurons Damaged by XO/HX

  • 신병철 (원광대학교 한의과대학 한방재활의학교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학교실)
  • Shin, Byung-Cheul (Dept. of Oriental Rehabilitation Medicine. College of Oriental Medicine. Won Kwong University) ;
  • Song, Yung-Sun (Dept. of Oriental Rehabilitation Medicine. College of Oriental Medicine. Won Kwong University)
  • 발행 : 2001.12.20
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초록

Objectives and Methods : To evaluate the mechanism of oxidative damage by xanthine oxydase(XO) and hypoxanthine(HX)-induced oxygen radicals, MTT assay and NR assay were carried out after the cultured mouse spinal sensory neurons were preincubated for 4 hours with various concentrations of XO/HX. And the amount of total protein. neurofilament EIA. lipid peroxidation and LDH activity were measured, to evaluate the protective effect of Herbar Chelidonii(HC) water extract on cultured spinal sensory neurons damaged by XO/HX. after the cultured mouse spinal sensory neurons were preincubated with various concentrations of HC water extract for 3 hours prior to exposure of XO/HX. Results : XO/HX decreased significantly the survival rate of the cultured mouse sensory neurons by NR assay and MTT assay In proportion to concentration and exposed time. In proportion to concentration and exposed time on cultured spinal sensory neurons, XO/HX showed the quantitative decrease of neurofilament by EIA. the decrease of total protein amount by SRB assay and the Increase of lipid peroxidation as well as LDH. HC showed the quantitative increase of neurofilament and total protein, but showed the decrease of lipid peroxidation and LDH activity against the neurotoxicity of XO/HX. Conclusions : From the above results, it is concluded that XO/HX have a neurotoxic effect on cultured spinal sensory neurons and that the herbs extract, such as HC, prevent the toxicity of XO/HX effectively in that they decrease lipid peroxidation and LDH activity.

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