The Korean Journal of Physiology and Pharmacology
- Volume 5 Issue 6
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- Pages.467-477
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- 2001
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
Regional Heterogeneity of Morphological Changes in Cultured Rat Astrocytes
- Won, Chung-Kil (Department of Pharmacology, College of Medicine, Korea University) ;
- Oh, Young-S. (Departments of Medicine and Neurobiology, University of Alabama at Birmingham)
- Published : 2001.12.21
Abstract
We examined astrocyte regional heterogeneity in their morphological changes in response to various stimuli. Astrocytes were cultured from six different neonatal rat brain regions including cerebral cortex, hippocampus, cerebellum, mid brain, brain stem and hypothalamus. Astrocyte stellation was induced by serum deprivation and the maximum stellation in different regional astrocytes was achieved after 2 h. After 24 h, in all astrocyte cultures, the level of stellation returned to their original level. Cerebellar or hypothalamic astrocytes were the most or the least sensitive, respectively, to serum deprivation. The order of maximum sensitivity to serum deprivation among different regional astrocytes was: cerebellum>mid
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