The Korean Journal of Physiology and Pharmacology
- Volume 5 Issue 3
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- Pages.223-230
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- 2001
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
The Effects of Melatonin on Cisplatin-Induced Renal Cortical Cell Injury in Rabbits
- Kim, Chung-Hui (Departments of Physiology and Biophysics, College of Midicine, Inje University) ;
- Han, Jin (Departments of Physiology and Biophysics, College of Midicine, Inje University) ;
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Kim, Na-Ri
(Departments of Physiology and Biophysics, College of Midicine, Inje University) ;
- Park, Ju-Hee (Departments of Physiology and Biophysics, College of Midicine, Inje University) ;
- Yang, Young-Churl (Department of Anatomy, College of Medicine, Inje University) ;
- Kim, Eui-Yong (Departments of Physiology and Biophysics, College of Midicine, Inje University)
- Published : 2001.06.21
Abstract
Melatonin, a pineal gland hormone, is believed to act as an antioxidant via the stimulation of radical detoxifying enzymes and scavenging of free radicals. In this study, effects of in vitro and in vivo treatments of melatonin on the cisplatin-induced lipid peroxidation, LDH release and plasma creatinine were determined in rabbit renal cortical cells. The level of malondialdehyde (MDA) was assayed as an index of lipid peroxidation and the level of LDH release as an indicator of cellular damage. In in vitro studies, cisplatin increased the levels of MDA and LDH release in a concentration-and time-dependent manner. Melatonin inhibited the cisplatin-induced lipid peroxidation and LDH release in a concentration-dependent manner. The minimal effective concentration of melatonin that significantly reduced the