The Korean Journal of Physiology and Pharmacology
- Volume 5 Issue 3
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- Pages.205-212
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- 2001
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
Increase of Peroxynitrite Production in the Rat Brain Following Transient Forebrain Ischemia
- Kim, Hee-Joon (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
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Kim, Seong-Yun
(Department of Pharmacology, College of Medicine, The Catholic University of Korea)
- Published : 2001.06.21
Abstract
It has been proposed that nitirc oxide is involved in the pathogenesis of cerebral ischemia-reperfusion. Because superoxide production is also enhanced during reperfusion, the cytotoxic oxidant peroxynitrite could be formed, but it is not known if this occurs following global forebrain ischemia-reperfusion. We examined whether peroxynitrite generation is increased in the vulnerable regions after forebrain ischemia-reperfusion. Transient forebrain ischemia was produced in the conscious rat by four-vessel occlusion. Rats were subjected to 10 or 15 min of forebrain ischemia. Immunohistochemical method was used to detect 3-nitrotyrosine, a marker of peroxynitrite production. 3-Nitrotyrosine immunoreactivity was enhanced in the hippocampal CA1 area 3 days after reperfusion. Furthermore, in rats subjected to ischemia for 15 min, this change was also observed in the lateral striatal region and the lateral septal nucleus
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