The Korean Journal of Physiology and Pharmacology
- Volume 5 Issue 3
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- Pages.199-204
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- 2001
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
Real Physiological Neuronal Responses Revealed by Gramicidin Perforated Patch Recording
- Akaike, Norio (Cellular and System Physiology, Graduate School of Medical Sciences, Kyushu University) ;
- Kakazu, Yasuhiro (Cellular and System Physiology, Graduate School of Medical Sciences, Kyushu University)
- Published : 2001.06.21
Abstract
In order to understand the phenomenon in a living cell correctly, it has been required to obtain intact responses from the cell membrane without disrupting the cytoplasmic circumstances. Gramicidin perforated patch configuration allows the electrical access to the whole cell with a minimal dialysis of cytoplasm and preventing the loss of native intracellular constituents, such as
Keywords
- Gramicidin perforated patch recording mode;
- Dissociated CNS neuron;
- Intracellular $Cl^-$ concentration;
- Intracellular $Cl^-$ regulation