BMB Reports
- 제33권3호
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- Pages.228-233
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- 2000
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- 1976-670X(eISSN)
The Kinetic Investigation of D-Hydroxyisovalerate Dehydrogenase from Fusarium sambucinum
- Lee, Chan (Department of Food Science and Technology, Chung-Ang University) ;
- Goerisch, Helmut (Institut fuer Biotechnilogie FG Technische Biochemie der Technische Universitaet Berlin) ;
- Zocher, Rainer (Institut fuer Biochemie und Molekulare Biologie, Technischen Universitaet Berlin)
- 투고 : 2000.01.21
- 심사 : 2000.03.11
- 발행 : 2000.05.31
초록
The steady-state investigation of the mechanism of Dhydroxyisovalerate dehydrogenase was performed in order to understand this type of kinetic patterns. The initial velocity was measured with various amounts of both substrates, NADPH and 2-ketoisovalerate. Double reciprocal plots gave patterns that conversed on or near the abscissa. Binding studies indicated that NADPH bound first to the enzyme. The product