Journal of Technologic Dentistry (대한치과기공학회지)
- Volume 22 Issue 1
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- Pages.145-152
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- 2000
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- 1229-3954(pISSN)
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- 2288-5218(eISSN)
Isolation of Methylotrophic Actionmycetes Capable of Producing Antagonistic Activity Against Oral Resident Bacteria and Screening of Mutants
구강상재균을 중심으로 항균력을 나타내는 메탄올 자화방선균의 분리 및 변이주 생산
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Park, Myoung-Ho
(Dept. of Dental Technology, Taegu Health College) ;
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Lee, Hwa-Sik
(Dept. of Dental Technology, Taegu Health College) ;
- Bae, Bong-Jin (Dept. of Dental Technology, Taegu Health College) ;
- Kim, Joung (Dept. of Dental Hygiene, Suwon Women's College)
- Published : 2000.12.10
Abstract
In order to select an effective antibiotic substance against oral resident bacteria, we were isolated from soil and texonomically analyzed. Seven hundred and eighteen strains were isolated on humic acid- vitamin agar(HV agar) and 220 strains were on methanol medium from three each paddy forest, field and riverside soil samples. So, during the screening of antibiotics from soil, we isolated microorganisms showing powerful antagonistic activity against oral resident bacteria. Microorganism was tested against 25 strains of bacteria, yeast and fungi. Among them, No. 248 strain exhibited the most strongly growth inhibition. So, the taxonomical analysis the isolated strain was found to be unknown Actinomyces sp. and was named No 248. A production of the antibiotics from No. 248 begins at the early exponential phase developed at the 72th hour under the optinum conditions. The property of No. 248 antimicrobial compound was very stable under acid(pH 3.0) and alkali(pH 10.0) treatment, but it was instable in heat treatment at
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