Clinical and Experimental Reproductive Medicine
- Volume 27 Issue 1
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- Pages.67-74
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- 2000
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Study on the Vitrification of Human Blastocysts: II. Effect of Vitrification on the Implantation and the Pregnancy of Human Blastocysts
인간 포배기 배아의 초자화 동결에 관한 연구: II. 초자화 동결이 포배기 배아의 착상 및 임신에 미치는 영향
- Kim, Su-Hee (Taegu-Maria Obstetrics/Gynecology) ;
- Lee, Sang-Won (Taegu-Maria Obstetrics/Gynecology) ;
- Lee, Ju-Hee (Taegu-Maria Obstetrics/Gynecology) ;
- Kang, Sang-Min (Taegu-Maria Obstetrics/Gynecology) ;
- Oh, Hee-Jeong (Taegu-Maria Obstetrics/Gynecology) ;
- Lee, Seoung-Min (Taegu-Maria Obstetrics/Gynecology) ;
- Lee, Seong-Goo (Taegu-Maria Obstetrics/Gynecology) ;
- Yoon, Hye-Gyun (Seoul-Maria Obstetrics/Gynecology & Maria Infertility Medical Institute) ;
- Yoon, San-Hyun (Seoul-Maria Obstetrics/Gynecology & Maria Infertility Medical Institute) ;
- Park, Se-Pill (Seoul-Maria Obstetrics/Gynecology & Maria Infertility Medical Institute) ;
- Song, Hai-Bum (Department of Animal Science, Taegu University) ;
- Lim, Jin-Ho (Seoul-Maria Obstetrics/Gynecology & Maria Infertility Medical Institute)
- 김수희 (대구마리아 산부인과) ;
- 이상원 (대구마리아 산부인과) ;
- 이주희 (대구마리아 산부인과) ;
- 강상민 (대구마리아 산부인과) ;
- 오희정 (대구마리아 산부인과) ;
- 이승민 (대구마리아 산부인과) ;
- 이성구 (대구마리아 산부인과) ;
- 윤혜균 (서울마리아 산부인과 & 마리아 기초의학 연구소) ;
- 윤산현 (서울마리아 산부인과 & 마리아 기초의학 연구소) ;
- 박세필 (서울마리아 산부인과 & 마리아 기초의학 연구소) ;
- 송해범 (대구대학교 축산학과) ;
- 임진호 (서울마리아 산부인과 & 마리아 기초의학 연구소)
- Published : 2000.03.30
Abstract
Objective: This study was conducted to investigate the effect of vitrification on the implantation and the pregnancy of human blastocysts. Method: The transfer of the frozen-thawed blastocysts by the slow freezing or vitrification was performed between January 1998 and July 1999. The zygotes derives from IVF were cocultured with cumulus cells in YS medium containing 20% hFF for 5days. Two or three of the best balstocysts produced on day 5 were transferred into the uterus, and then supernumerary blastocysts were randomly divided into two groups. One was frozen by slow freezing and the other was frozen by vitrification method. The slow freezing procedure was performed in two steps (5% glycerol and 9% glycerol + 0.2 M sucrose for 10 min, respectively) using programmed freezer (