Clinical and Experimental Reproductive Medicine
- Volume 27 Issue 1
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- Pages.23-29
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- 2000
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Human Amniotic Fluid Induces Spontaneous Hardening of the Zona Pellucida of Mouse Immature Oocytes During Maturation In Vitro
인간양수에 의한 생쥐 난자 투명대의 정자수용능력 억제의 관찰
- Park, Kee-Sang (Department of OB/Gyn, Kyungpook National University Hospital, Department of Animal Science, Taegu University) ;
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Lee, Taek-Hoo
(Department of OB/Gyn, Kyungpook National University Hospital) ;
- Song, Hai-Bum (Department of Animal Science, Taegu University) ;
- Chun, Sang-Sik (Department of OB/Gyn, Kyungpook National University Hospital)
- 박기상 (경북대학교병원 산부인과학교실, 대구대학교 축산학과) ;
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이택후
(경북대학교병원 산부인과학교실) ;
- 송해범 (대구대학교 축산학과) ;
- 전상식 (경북대학교병원 산부인과학교실)
- Published : 2000.03.30
Abstract
Objective: Zona pellucida (ZP) has been thought to be the barrier of egg to sperm penetration before and after fertilization. The phenomenon of ZP hardening has been considered as a post-fertilization event until now, and it is generally accepted that it is caused by the secretory products of cortical granules released during the cortical reaction. Hardening of ZP could occur "spontaneously" in mammalian oocytes in standard culture conditions, and that it is probably not a consequence of cortical reaction. The purpose of our study was to investigate the effect of human amniotic fluid (HAF) on nuclear maturation (NM) and fertilization ability of mouse immature oocytes. Methods: HAF was obtained from patients undergoing amniocentesis at