Clinical and Experimental Reproductive Medicine
- Volume 27 Issue 3
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- Pages.267-273
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- 2000
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Effect of Hypo-osmotic Swelling (HOS) Test on Subsequent Post-thaw Testicular Spermatozoa
고환조직 동결-융해 후 회수된 고환 정자에 대한 Hypo-osmotic Swelling (HOS) Test의 효과
- Park, Yong-Seog (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine) ;
- Lee, Hyoung-Song (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine) ;
- Song, Sang-Jin (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine) ;
- Kim, Jeong-Wook (Laboratory of Reproductive Biology & Infertility, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine) ;
- Kang, Inn-Soo (Department of OB/GYN, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine) ;
- Seo, Ju-Tae (Department of Urology, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine)
- 박용석 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
- 이형송 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
- 송상진 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
- 김정욱 (성균관대학교 의과대학 삼성제일병원 생식생물학 및 불임연구실) ;
- 강인수 (성균관대학교 의과대학 삼성제일병원 산부인과) ;
- 서주태 (성균관대학교 의과대학 삼성제일병원 비뇨기과)
- Published : 2000.09.30
Abstract
Objectives: We have previous reported that thawed testicular sperm and sperm extracted from seminiferous tubule could achieved optimal fertilization and pregnancy in azoospermic patients. However, thawed testicular sperm did not show motility in many cases. Therefore we studied viability of immotile sperm extracted from frozen-thawed seminiferous tubule using hypo-osmotic swelling (HOS) test and eosin-Y test. Materials and Methods: After sperm extraction using for ICSI, the remained sections of seminiferous tubules were frozen with a computerized freezer. For thawing and preparation of testicular sperm, the seminiferous tubules were thawed by removing from