Journal of Korean Neurosurgical Society
- Volume 29 Issue 10
- /
- Pages.1283-1288
- /
- 2000
- /
- 2005-3711(pISSN)
- /
- 1598-7876(eISSN)
Bad Translocation to Mitochondria with Bcl-XL Traced in-vivo by Using GFP
GFP를 이용하여 in-vivo에서 추적한 Bad와 Bcl-XL의 Mitochondria 이동
- Yoon, Soo Han (Department of Neurosurgery, Ajou University School of Medicine) ;
- Kim, Jin Young (Department of Neurosurgery, Ajou University School of Medicine) ;
- Park, Seung Woo (Department of Neurosurgery, Ajou University School of Medicine) ;
-
Ahn, Young Hwan
(Department of Neurosurgery, Ajou University School of Medicine) ;
- Ahn, Young Min (Department of Neurosurgery, Ajou University School of Medicine) ;
- Cho, Ki Hong (Department of Neurosurgery, Ajou University School of Medicine) ;
-
Cho, Kyung Gi
(Department of Neurosurgery, Ajou University School of Medicine)
- 윤수한 (아주대학교 의과대학 신경외과학교실) ;
- 김진영 (아주대학교 의과대학 신경외과학교실) ;
- 박승우 (아주대학교 의과대학 신경외과학교실) ;
-
안영환
(아주대학교 의과대학 신경외과학교실) ;
- 안영민 (아주대학교 의과대학 신경외과학교실) ;
- 조기홍 (아주대학교 의과대학 신경외과학교실) ;
-
조경기
(아주대학교 의과대학 신경외과학교실)
- Received : 1999.12.18
- Accepted : 2000.03.07
- Published : 2000.10.28
Abstract
Objectives : The subcellular locations of Bad, Bid, Bax and Bcl-XL change during apoptosis and this change is important for the regulation of cell death. The purpose this study was to elucidate binding of Bad with Bcl-XL in vivo Methods : We mads Bad with Green Fluorescent Protein(GFP) using PCR method. We transfected and overexpressed GFP-Bad with or without Bcl-XL cotransfection in living COS-7 cell. Results : Bad and Bcl- XL bind one another in healthy living cells and this association controled mitochondrial docking. In the absence of Bad-XL, Bad was mainly cytosolic and partially bound to mitochondria. Upon coexpression of Bad and Bcl-XL, most of Bad translocated to mitochondria. These should suggest that Bad binds to the mitochondrial and cytoplasmic forms of Bcl-XL and Bad bound to cytoplasmic Bcl-XL translocates to mitochondria. These in vivo findings confirm that Bad make a complexes with Bcl- XL and cause mitochondrial translocation of Bad-Bcl-XL complex.