원예과학기술지 (Horticultural Science & Technology)
- 제18권6호
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- Pages.802-807
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- 2000
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- 1226-8763(pISSN)
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- 2465-8588(eISSN)
Ri t-DNA로 형질전환된 당근 뿌리를 이용한 Arbuscular 균근균의 기내증식
In vitro Propagation of Arbuscular Mycorrhizal Fungi using Ri t-DNA Transformed Carrot Roots
- Cho, Ja Yong (Dept. of Ornamental Horticulture, Namdo Provincial College) ;
- Sohn, Bo-Kyoon (Dept. of Agrochemistry, Sunchon Nat'l Univ) ;
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Lee, Hyo-Yeon
(Dept. of Agrochemistry, Sunchon Nat'l Univ) ;
- Chung, Soon-Ju (Dept. of Horticulture, Chonnam Nat'l Univ)
- 발행 : 2000.12.31
초록
Agrobacterium rhizogenes의 Ri t-DNA로 형질전환된 당근의 모상근에 Glomus sp.를 감염시킨후 arbuscular 균근균 포자를 Modified Strullu & Romand 배지상에서 12주 동안 기내증식하였다. 기내증식을 위한 접종원으로는 포자보다는 균근감염 뿌리가 더 좋았다. 당근 모상근과 arbuscular 균근균의 대치배양으로 증식된 Glomus sp.의 포자는 직경 약
This study was conducted to propagate the arbuscular mycorrhizal fungi in vitro using the hairy root of carrot transformed by Agrobacterium rhizogenes with Ri t-DNA. Mycorrhizal spores and roots in sudangrass plants were wet-sieved, surface-sterilized and inoculated onto the hairy root of carrot on the Modified Strullu & Romand (MSR) medium. The mycorrhizal spores of Glomus sp. propagated in vitro for 12 weeks was about