Enhanced Proliferation and Altered Intracellular Zinc Levels in Early- and Late-Passage Mouse Aorta Smooth Muscle Cells

  • Moon Sung-Kwon (Univ. of Texas Medical Branch, Div. of Cardiology and Sealy, Center for Molecular Cardiology) ;
  • Ha Sang-Do (Dept. of Industry and Technology, Korea Health Industry Development Institute)
  • 발행 : 2000.01.01

초록

Cell growth and DNA synthesis were studied from a cultured early- and late- pas- sage mouse aorta smooth muscle cell (MASMC) because the proliferation of vascular smooth muscle cell (VSMC) is a key factor in development of atherosclerosis. In this study, the cells were cultured in fetal bovine serum (FBS) and stimulated by growth factors such as thrombin and platelet-derived growth factor-BB (PDGF-BB). Compared to the number of early-passage MASMC (passage 3 to 9) the number of late-passage MASMC (passage 30 to 40) in a normal serum state was increased 2 fold at Day 1, 3 and 6 in culture, respectively. Incorporation of $[^3H]$ thymidine into DNA induced by serum, PDGF and thrombin in late-passage MASMC was greater than those in early-passage MASMC. We also examined whether intracellular zinc levels would be an aging factor or not. The intracellular zinc level in early- and late-passage MASMC was monitored by using the zinc probe dye N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide. It is interested that late-passage MASMC increased the intracellular fluorescence level of zinc, more than the early passage MASMC did. The alterations of intracellular zinc level occur concurrently with changes in MASMC proliferation rate during aging. This data suggest that the age-associated changes in zinc concentrations may provide a new in vitro model for the study of smooth muscle cell differentiation.

키워드

참고문헌

  1. Exp. Mol. Pathol. v.64 Enhanced proliferation and migration and altered cytoskeletal proteins in early passage smooth muscle cells from young and old rat aortic explants Li, Z.;H. Cheng;W. J. Lederer;J. Froehlich;E. G. Lakatta
  2. Exp. Mol. Pathol. v.58 Human neonatal and adult vascular smooth muscle cells in culture Fujita, H.;K. Shimokado;C. Yutani;S. Takaichi;J. Masuda,
  3. Differentiation v.49 Cultured aortic smooth muscle cells from newborn and adult rats show distinct cytoskeletal features Bochaton-Piallat;M. L.;F. Gabbiani;P. Ropraz;G. Gabbiani
  4. J. Anat. v.113 The growth and development of the rat aorta. morphological aspects Berry, C. L.;T. Looker;J. Germain
  5. Physiol. Rev. v.59 The smooth muscle cells in culture Chamley-Campbell, J., G. R. Campbell, and R. Ross
  6. J. Clin. Invest. v.73 Actin expression in smooth muscle cells of rat aortic intimal thickening, human atheromatous plaque and cultured rat aortic media Gabbiani, G.;O. Kocher;W. S. Bloom;J. Vanderckhove;K. Weber
  7. Lab. Invest. v.17 The aortic tunica media in growing rats studied with the electron microscope Cliff, W. J.
  8. J. Cell Biol. v.89 Phenotype-dependent response of cultured aortic smooth muscle to serum mitogen Chamley-Campbell, J.;G. R. Campbell;R. Ross
  9. Circ. Res. v.74 Developmentally timed expres-sion of a growth phenotype in vascular smooth muscle cell Cook, C. L.;M. C. M. Weiser;P. E. Schwartz;C. J. Jones;R. A. Majack
  10. Physiol. Rev. v.73 Cardiovascular regulatory mechanisms in advanced age. Lakatta, E. G.
  11. Science v.273 Oxidative stress, caloric restriction, and aging Sohal, R. and R. Weindruch
  12. Nature Genet. v.13 Genetic analysis of aging: role of oxidative damage and environmental stresses Martin, G.;S. Austad;Y. Johnson
  13. Physiol. Rev. v.78 The free radical theory of aging matures Beckman, K.;B. N. Ames
  14. Proc. Natl. Acad. Sci. v.94 Mitochondrial decay in hepatocytes from old rats: membrane potential declines, heterogeneity and oxidants increase Hagen, T. M.;D. L. Yowe;J. C. Bartholomew;C. M. Wehr;K. L. Do;J. Y. Park;B. N. Ames
  15. Neuroscience v.89 Zinc-induced cortical neuronal death with feature of apoptosis and necrosis: mediation by free radicals Kim, Y. H.;E. Y. Kim;B. J. Gwag;S. Sohn;J. Y. Koh
  16. J. Biol. Chem. v.257 The mechanism of the insulin-like effects of ionic zinc May, J. M.;C. S. Contoreggi
  17. Arch. Biochem. Biophys. v.334 Direct reaction of H2O2 with sulfhydryl groups in HL-60 cells: zinc-metallothionein and other sites Quesada, A. R.;R. W. Byrnes;S. O. Krezoski;D. H. Petering
  18. Am. J. Physiol. v.276 Metallothionein-overexpressing neonatal mouse cardiomyocytes are resistant to H2O2 toxicity. Wang, G. W.;D. A. Schuschke;Y. J. Kang
  19. Brain Res. (in press) His-brains. Suh, S. W.;K. B. Jensen;M. S. Jensen;D. S. Silva;P. J. Kesslak;G. Danscher;C. J. Frederickson
  20. J. Cell Biol. v.92 Functional angiotensin II receptors in cultured vascular smooth muscle cells Gunther, S.;R. W. Alexander;W. J. Atkinson;M. A. Gimbrone, Jr.
  21. Hypertension v.13 Angiotensin II-stimulated protein synthesis in cultured vascular smooth muscle cells Berk, B. C.;V. Vekshtein;H. M. Gordon;T. Tsuda
  22. J. Clin. Invest. v.98 Downregulation of vascular endothelial growth factor receptors by tumar necrosis factor-alpa in cultured human vascular endothelial cells Patterson, C.;M. A. Perrella;W. O. Endege;M. Yoshizumi; M. E. Lee;E. Haber
  23. Brain Res. v.480 Translocation of zinc may contribute to seizureinduced death of neurons Frederickson, C. J.;M. D. Hernandez;J. F. McGinty
  24. N. Engl. J. Med. v.330 The emerging concept of vascular remodeling Gibbons, G. H.;V. J. Dzau
  25. Am. Geriat. Soc. v.8 Prolongation of life: role of free radical reaction in aging. Harman, D.
  26. J. Biol. Chem. v.274 Ras induces senescence by altering the intracellular levels of reactive oxygen species. Lee, A. C.;B. E. Fenster;H. Ito;K. Takeda;N. S. Bae;T. Hirai;Z. X. Yu;V. J. Ferrans;B. H. Howard;T. Finkel
  27. Eur. J. Neurosci. v.11 Zn2+ entry produces oxidative neuronal necrosis in cortical cell cultures. Kim, E. Y.;J. Y. Koh;Y. H. Kim;S. H. Sohn;E. H. Joe;B. J. Gwag