Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Selection of Cryoprotectant for the Cryopreservation of Trochophores and Early D-shaped Larvae of Surf Clam, Spisula sachalinensis

북방대합, Spisula sachalinensis 담륜자와 초기 D상 유생의 냉동보존을 위한 동해방지제의 선택

  • KIM Young Sin (Department of Aquaculture, Pukyong National University) ;
  • CHOI Youn Hee (Department of Aquaculture, Pukyong National University) ;
  • LEE Jeong Yong (Kangnung Hatchery, National Fisheries Research and Development Institute) ;
  • CHANG Young Jin (Department of Aquaculture, Pukyong National University)
  • 김영신 (부경대학교 수산과학대학 양식학과) ;
  • 최윤희 (부경대학교 수산과학대학 양식학과) ;
  • 이정용 (국립수산진흥원 강릉수산종묘시험장) ;
  • 장영진 (부경대학교 수산과학대학 양식학과)
  • Published : 2000.11.01
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Abstract

This study was performed to find out the cryoprotectants for cryopreservation of trochophores and early D-shaped larvae of surf clam, Spisula sachalinensis. Dimethyl sulfoxide (DMSO), ethylene glycol, 1,2-propanediol and methanol were used as cryoprotectant Each cryoprotectant was made to 1.0 M, 2.0 M, 3.0 M with dilution of 0.2 M fructose and 0.2 M sucrose. The trochophoers and early D-shaped larvae were immersed in each preparation for 10 minutes to reach equilibration and cryopreserved in liquid nitrogen. Survival rates of post-thawed trochophores and early D-shaped larvae in 2.0 M DMSO with 0.2 M sucrose were the highest as $91.4{\%}\;and\;78.9{\%}$, respectively.

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