Characteristics of Tyrosinase Inhibitory Extract from Ecklonia stolonifera

  • Park Douck-Choun (Faculty of Food and Biotechnology, Pukyong National University) ;
  • Ji Cheong-Il (Faculty of Food and Biotechnology, Pukyong National University) ;
  • Kim Sang-Ho (Faculty of Food and Biotechnology, Pukyong National University) ;
  • Jung Kyoo-Jin (Department of Marine Food Industry, Provincial College of Namdo) ;
  • Lee Tae-Gee (Department of Marine Food Industry, Provincial College of Namdo) ;
  • Kim In-Soo (Department of Food Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Park Yeung-Ho (Faculty of Food and Biotechnology, Pukyong National University) ;
  • Kim Seon-Bong (Faculty of Food and Biotechnology, Pukyong National University)
  • Published : 2000.12.01

Abstract

Tyrosinase inhibitory activities of 14 kinds of seaweed, Ecklonia stolonifera, Ecklonia cava, Undaria pinnatiflda, Laminaria japonica, Sargassum fulvellum, Sargassum miyabei, Sargassum thunbergii, Porphyra yezoensis, Gracilaria verrucosa, Carpopeltis affinis, Pachymeniopsis elliptica, Gelidium amansii, Codium fragile and Ulva pertusa were determined using commercially available mushroom tyrosinase in an in vitro assay system. The $1\%$ (w/v) methanol extract from E. stolonifera showed the highest tyrosinase inhibitory activity of $79.0\%$, electron donating activity of $79.0\%$ and total phenol content of 3.75 mg/100g. Ethyl acetate-methanol-water (7 : 2 : 0.2, v/v) fraction $(0.5\%,\;w/v)$ isolated from the methanol extract showed tyrosinase inhibitory activity of $75.9 \%$, electron donating activity of $88.1 \%$ and total phenol content of 4.38 mg/100g. Tyrosinase inhibitory activity was closely associated with total phenol content (R = 0.99) and electron donating activity (R=0.99). Maximum absorption wavelength of the fraction was 218nm and that of phenolic compounds showed about a range from 210 to 220nm. The inhibition mode of the fraction was noncompetitive inhibition.

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