대한수의학회지 (Korean Journal of Veterinary Research)
- 제40권1호
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- Pages.130-137
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- 2000
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
개 심장사상충(Dirofilaria immitis) 진단을 위한 항원성 조사 및 단크론항체 생산
Studies on antigenicity and production of monoclonal antibody for diagnosis of canine heartworm(Dirofilaria immitis)
- Lee, Cheol-soon (College of Veterinary Medicine, Chungbuk National University) ;
- Jee, Cha-ho (College of Veterinary Medicine, Chungbuk National University)
- 투고 : 1999.12.22
- 발행 : 2000.03.25
초록
In order to diagnose canine heartworm infection by antigen capture ELISA, the crude somatic(S), partial somatic(below 45kDa) and excretory/secretory(E/S) antigen of adult heartworm were identified and the antigenicity was examined by silver stain, immunoblot and ELISA. Then, production of monoclonal antibody to specific antigen carried out in this experiment. The bands to S antigen and E/S antigen were recognized between 10 and 200kDa and common bands were recognized strongly 14, 18, 28, 43kDa by silver stain. By western blot analysis, fractions to S antigen were recognized 14, 16, 18, 20, 24, 28, 32, 43, 50, 55kDa, etc. and only a 14kDa to E/S antigen in positive sera which were positive in modified Knott's test and necropsy. In ELISA, the positive sera reacted to antigens(SA,
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