대한수의학회지 (Korean Journal of Veterinary Research)
- 제40권1호
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- Pages.101-109
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- 2000
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
오리 간염 바이러스의 분리와 국내 분리주의 약독화
Isolation of duck hepatitis virus and it's attenuation in chicken embryos
- Sung, Haan-woo (National Veterinary Research and Quarantine Service, Ministry of Agriculture & Forestry) ;
- Kim, Jae-hong (National Veterinary Research and Quarantine Service, Ministry of Agriculture & Forestry)
- 투고 : 2000.02.16
- 발행 : 2000.03.25
초록
Duck viral hepatitis is an acute, highly infectious viral disease of young dacklings aged from two days to three weeks. The significant lesion associated with the disease was enlarged liver including necrotic foci and numerous hemorrhagic spots. We have isolated five strains of duck hepatitis virus (DHV) from field cases showing about 20% mortality with a sign of opisthotonos. When a-day-old ducklings were intramuscularly inoculated with one of the isolates, 92% of the birds were died within 5 days. We attempted to develop an attenuated strain of duck hepatitis virus (DHV) using one of the isolates by serial chicken embryo passages. The propagation of DHV in chicken embryos was carried 140 passages. The virus titer increased gradually from the