대한수의학회지 (Korean Journal of Veterinary Research)
- 제40권1호
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- Pages.63-71
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- 2000
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Pasteurella multocida type A의 lipopolysaccharide-protein 복합체의 특성
Characterization of a lipopolysaccharide-protein complex of type A Pasteurella multocida
- Ryu, Hyo-ik (Department of Enteric Infections, Walter Reed Army Institute of Research) ;
- Kim, Chul-joong (College of Veterinary Medicine, Chungnam National University)
- 투고 : 2000.01.05
- 발행 : 2000.03.25
초록
An immunogenic, high molecular weight lipopolysaccharide (LPS)-protein complex isolated from a potassium thioncyanate extract of a Pasteurella multocida (P multocida ; strain P-2383, capsular type A and somatic type 3) was characterized. Chemical analysis of the complex by gas chromatography on a capillary column demonstrated that this complex contained most of the chemical constituents characteristic of LPS extracted by the phenol-water methed from the whole bacterium. However, there was proportionately more carbohydrate than fatty acid in the complex in contrast to LPS in which fatty acid seemed to be in excess. When toxicity of the complex was evaluated in 10-day-old chicken embryos, the complex was less toxic (