Korean Journal of Veterinary Research (대한수의학회지)
- Volume 40 Issue 1
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- Pages.42-48
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- 2000
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Isolation of akabane virus and its molecular diagnosis by reverse transcription polymerase chain reaction
아까바네 바이러스의 분리 및 RT-PCR 진단법에 관한 연구
- Cho, Jae-jin (National Veterinary Research and Quarantine Service) ;
- Lee, Chung-gil (College of Veterinary Medicine, Chonnam National University) ;
- Park, Bong-kyun (College of Veterinary Medicine, Seoul National University) ;
- Chang, Chung-ho (National Veterinary Research and Quarantine Service) ;
- Chung, Chung-won (National Veterinary Research and Quarantine Service) ;
- Cho, In-soo (National Veterinary Research and Quarantine Service) ;
- An, Soo-hwan (National Veterinary Research and Quarantine Service)
- 조재진 (국립수의과학검역원) ;
- 이정길 (전남대학교 수의과대학) ;
- 박봉균 (서울대학교 수의과대학) ;
- 장정호 (국립수의과학검역원) ;
- 정정원 (국립수의과학검역원) ;
- 조인수 (국립수의과학검역원) ;
- 안수환 (국립수의과학검역원)
- Received : 1999.11.30
- Published : 2000.03.25
Abstract
Akabane disease is transmitted through mosquitoes in cattle, sheep and goats. It shows congenital abnormalities including encephalomyetitis, hydranencephaly, neurogenic arthrogryposis, and deformed neonatal calves. Akabane viruses, 93FMX and K-9 strain, were isolated from fetal matrix of aborted cow and blood of healthy cow, respectively. S gene sequences of 93FMX and K-9 showed 100% homology with that of OBE-1 strain isolated in Japan. Based upon our sequencing data, we synthesized specific primers for PCR diagnosis. Using these primers, we were able to amplify the S gene of Akabane virus not only from the culture fluid of Vero cells but also from the brain tissue of suckling mouse inoculated with, Akabane virus. These PCR products were confirmed by Southern blot hybridization. Not only the sensitivity of PCR test was high enough to detect the viruses of