대한수의학회지 (Korean Journal of Veterinary Research)
- 제40권4호
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- Pages.701-706
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- 2000
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
양돈용 생균제 개발을 위한 유산균주 선발
In vitro selection of lactic acid bacteria for probiotic use in pigs
- 변재원 (충북대학교 수의과대학) ;
- 김경태 ((주)한국야쿠르트 중앙연구소) ;
- 배형석 ((주)한국야쿠르트 중앙연구소) ;
- 백영진 ((주)한국야쿠르트 중앙연구소) ;
- 이완규 (충북대학교 수의과대학)
- Byun, Jae-won (College of Veterinary Medicine, Chungbuk National University) ;
- Kim, Gyung-tae (Korea Yakult Ltd. R & D Center) ;
- Bae, Hyoung-suk (Korea Yakult Ltd. R & D Center) ;
- Baek, Voung-jin (Korea Yakult Ltd. R & D Center) ;
- Lee, Wan-kyu (College of Veterinary Medicine, Chungbuk National University)
- 심사 : 2000.10.27
- 발행 : 2000.12.20
초록
This study was carried out to select the lactic acid bacteria(Lactobacillus, Streptococcus and Bifidobacterium) and yeast for probiotic use in pigs. Acid-tolerant 536 strains were isolated from the feces of 30 pigs. To select useful strains, the first screened strains were treated with strong acid solution(pH 2.5 to 3.0) for 3 hours and subsequentely treated with the anaerobic diluent solution containing 0.15% Oxgall for 3 hours. Among these strains, 151 strains showed strong tolerance to both acid and bile. Lactobacillus and Streptococcus tolerant to the acid and bile were treated with heat at
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