Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

Histopathologic study on the microvascular anastomosis of streptozotocin induced diabetic rats.

스트렙토조토신으로 유도된 당뇨백서에서 미세혈관문합술후 혈관의 조직병리학적 연구

  • Park, Sung-Jin (Dept. of Oral and Maxillofacial surgery, College of Medicine, Dong-A University) ;
  • Shin, Sang-Hun (Dept. of Oral and Maxillofacial surgery, College of Medicine, Dong-A University) ;
  • Jung, In-Kyo (Dept. of Oral and Maxillofacial surgery, College of Dentistry, Pusan National University)
  • 박성진 (동아대학교 의과대학 구강악안면외과) ;
  • 신상훈 (동아대학교 의과대학 구강악안면외과) ;
  • 정인교 (부산대학교 치과대학 구강악안면외과학교실)
  • Published : 2000.06.30
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Abstract

Purpose : The purpose of this study is to estabilish clinical guidance of microvascular anastomosis in diabetic patients. This study was performed with experimental microvascular anastomosis in streptozotocin induced diabetic rats and observed histopathologic change and endohelial healing process. Materials and Methods : 70 Sprague-Dawley rats, weighting 200 to 250grams, were used for the experiment. 35 induced diabetic rats with streptozotocin and 35 control group were selected. After end-to-end carotid artery microvascular anastomosis was done, the experimental rats were sacrificed at different time interval (1st day, 3rd day, 1st week, 2nd, 4th, 6th and 8th week) for histologic examination. Light microscope observation was used in this study. Results : 1. Histopathologic changes are nearly the same healing process in two groups. But period of tissue reaction was faster in the control than diabetic group. 2. In endotheliall healing, control group started at 1 week after and completed at 4 weeks after, but diabetic group was observed partially at 4 weeks after and complete healing was not observed still at 8 weeks after. 3. In subintimal hyperplasia, control group was observed at 6 weeks after but diabetic group was observed at 6 weeks after and partially at 8 weeks after. 4. All groups showed severe inflammatory response in the early period. This respond is decreased at 2 weeks after in control group but still remained at 8 weeks after in the diabetic group. 5. In media, inflammatory response and degeneration were observed in early period. Regeneration of smooth muscle cell was observed at 1 week after in control group but 4 weeks after in the diabetic group. Conclusions : As the results of study, it could be thought that vascular regeneration process was not failured but delayed in the diabetes. It was considered that diabetes mellitus was not absolute contraindication of microvascular anastomosis.

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