HPTLC 및 GC/MS를 이용한 유해화학물질의 스크리닝에 관한 연구

A study on the screening of toxic materials by HPTLC and GC/MS

  • 투고 : 1999.10.15
  • 발행 : 2000.02.25

초록

생체시료 및 환경시료에서 법과학분야에서 많이 다루어지는 유해화학물질의 효과적인 동시 스크리닝을 위해 HPTLC 및 GC/MS를 이용하여 분석한 후 데이터베이스를 구축하여 검색자료로 활용하고자 하였다. HPTLC 시스템을 이용하여 농약류 160종, 화공약품류 34종, 폭약류 39종 총 233종의 표준품에 대해 $R_f$치 및 UV 스펙트럼을 측정, database화 하였으며 또한 GC/MS를 이용하여 농약류 112종, 화공약품류 31종, 폭약류 17종, 휘발성 유기화합물류(VOCs) 57종 총 217종의 표준품에 대해 RT 및 특성질량이온들을 측정, dataobase화 하여 library를 구축하였다.

To perform an effective screening for toxic materials of forensic interest detected in high profile criminal case in biological and environmental samples, we tried to construct a searchable computerized database using HPTLC(High Performance Thin Layer Chromatography) and GC/MS. Retardation factor($R_f$) values and UV spectral data of HPTLC were investigated for 160 pesticides, 34 chemicals and 39 explosives of standard grade. The data were compiled in a library. We also analyzed 112 pesticides, 31 chemicals and 17 explosives and 57 volatile organic compounds(VOCs) by GC/MS. The data for RT and characteristic mass ions were also compiled in a library.

키워드

참고문헌

  1. DFG Commission for Clinical Toxicological Analysis., Report 18 Gas Chromatographic Retention Indices of Toxicologically Relevant Substances on Packed or Capillary Columns with Dimethylsilicone Stationary Phase R.A. de Zeeuw;J.P. Franke;H.H. Maurer;K. Pfleger
  2. Can. Soc. Forens. Sci. J. v.19 no.2 Evaluation of a Screening Procedure for Basic and Neutral Drugs : n-Butyl Chloride Extraction and Megabore Gas Chromatography M.E. Sharp
  3. 분석과학 v.5 no.1 유기화합물의 체계적인 분석 김택제
  4. Can. Soc. Forens. Sci. J. v.31 no.1 Capillary Zone Electrophoresis in a Comprehensive Screen for Drugs of Forensic Interest in Whole Blood J.C. Hudson;M. Gdin;M.J. Malcolm;C.F. Whiting
  5. J. Microcol. Sep. v.2 Capillary Electrophoresis Applied to the Analysis of Pharmaceutical Compounds A. Wainright
  6. 보건복지부 고시 제1995-45호 생약의 잔류농약 허용 기준 및 시험방법 보건복지부
  7. 법과학 유영찬
  8. Anal. Chem. v.67 no.3 Screening of 265 Pesticides in Water by Thin-Layer Chromatography with Automated multiple Development S. Butz;H.-J. Stan
  9. HPTLC Quantitative Analysis of Pharmaceutical Formulations P.D. Sethi
  10. 농약총람 윤채혁
  11. Forensic Sci. Int. v.51 Solid Phase Extraction Method for Rapid Isolation and Clean-Up of Some Synthetic Pyrethroid Insecticides from Human Urineand Plasma J. Liu;C. Fan
  12. Forensic Sci. Int. v.99 Conditions of Solid-Phase Extraction for the Mixture of Organophosphates and Synthetic Pyrethroids in Human Body Fluids J. Liu;O. Suzuki