The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Identification and Phylogenetic Relationship of Dermatophytes Based on RFLP Analysis and Nucleotide Sequence of Internal Transcribed Spacer (ITS)1 in Nuclear Ribosome DNA

ITS-RFLP와 ITS1 염기서열 분석에 의한 피부사상균의 동정과 계통적 유연관계

  • Choi, Yeon-Hwa (Laboratory of Nosocomial Pathogens, Department of Microbiological Diseases, National Institute of Health) ;
  • Lee, Yeong-Seon (Laboratory of Nosocomial Pathogens, Department of Microbiological Diseases, National Institute of Health) ;
  • Yoo, Jae-Il (Laboratory of Nosocomial Pathogens, Department of Microbiological Diseases, National Institute of Health) ;
  • Kim, Bong-Su (Laboratory of Nosocomial Pathogens, Department of Microbiological Diseases, National Institute of Health)
  • 최연화 (국립보건원 세균질환부 병원감염과) ;
  • 이영선 (국립보건원 세균질환부 병원감염과) ;
  • 유재일 (국립보건원 세균질환부 병원감염과) ;
  • 김봉수 (국립보건원 세균질환부 병원감염과)
  • Published : 2000.02.28
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Abstract

ITSI-5.8S-ITSII rDNA region was amplified from the reference strains and clinical isolates with ITS1 and ITS4 primers. These primers amplified DNA fragments of 550 bp in Microsporum audouinii and Trichophyton violaceum, 700 bp in Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum, and Trichophyton tonsurans, and 750 bp in Microsporum ferreugineum and Microsporum canis. The restriction enzyme patterns of PCR products digested with 13 restriction enzyme including PstI were distint among the genera, whereas identical in the same species. Examination of the ITS (Internal Transcribed Spacers)1 nucleotide sequence revealed that there was the genetic difference in each genera and species. Phylogenetic relationship among each species showed that the Trichophyton mentagrophytes was more closely related Trichophyton tonsurans than Trichophyton rubrum, and Microsporum gypseum was less related than Microsporum spp..

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