The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Molecular Typing of Acinetobacter Baumannii Strains by Randomly Amplified Polymorphic DNA (RAPD) Analysis

Randomly Amplified Polymorphic DNA (RAPD) 분석에 의한 Acinetobacter Baumannii 균주의 유전형 분류

  • Oh, Jae-Young (Department of Microbiology, Seonam University College of Medicine) ;
  • Cho, Jae-Wee (Department of Microbiology, Seonam University College of Medicine) ;
  • Park, Jong-Chun (Department of Microbiology, Seonam University College of Medicine) ;
  • Lee, Je-Chul (Department of Microbiology, Seonam University College of Medicine)
  • 오재영 (서남대학교 의과대학 미생물학교실) ;
  • 조재위 (서남대학교 의과대학 미생물학교실) ;
  • 박종천 (서남대학교 의과대학 미생물학교실) ;
  • 이제철 (서남대학교 의과대학 미생물학교실)
  • Published : 2000.04.30
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Abstract

Acinetobacter baumannii strains are emerging pathogens of the nosocomial infection with an increasing frequency in recent years. The therapeutic difficulty due to the wide spread of multiple resistant strains was major problem in A. baumannii infection. It seems likely that high frequency of A. baumannii infection will be increasing epidemiological importance in the future. However, the current limited understanding of the epidemiology of A. baumannii infections is caused by lack of a rapid and practical method for the molecular characterization of A. baumannii strains. This study was undertaken to determine molecular types and genetic similarity among A. baumannii strains isolated from four hospitals by RAPD analysis. Eighty-five strains, including 40 from Chunnam University Hospital, 27 from Dankook University Hospital, 15 from Yonsei University Hospital, and 3 from Seonam University Hospital, were classified into three molecular types. Molecular type II was the most common pattern and included 72 strains. All strains from Dankook University Hospital and 40 strains from Chunnam University Hospital belonged to molecular type I or II. A. baumannii strains form Yonsei University Hospital were very distant similarity values. The range of genetic similarity values among 85 strains of A. baumannii was 0.26 to 1.00. Although phenotypes including biotype and antimicrobial resistance pattern of A. baumannii strains were same or very similar to each other, their RAPD patterns were quite different. Typing with phenotypes was found to be less reliable than molecular typing by RAPD analysis. These results suggest that RAPD analysis provides rapid and simple typing method of A. baumannii strains for epidemiological studies. This work is the first epidemiological report of A. baumannii infections in Korea and it is hoped that results of this work may contribute to a better understanding of the clinical importance and epidemiology of A. baumannii strains.

Keywords

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