The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Clonal Analysis of Methicillin-Resistant Staphylococcus aureus Strains in Korea

  • Kim, Jung-Min (Department of Microbiology, College of Medicine, Dankook University) ;
  • Seol, Sung-Yong (Department of Microbiology, School of Medicine, Kyungpook National University) ;
  • Cho, Dong-Taek (Department of Microbiology, School of Medicine, Kyungpook National University)
  • Published : 2000.06.30
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Abstract

In this study, the distribution of the mec regulator genes and the presence of the mutation in mecI gene and mec promoter region among 50 MRSA clinical isolates derived from a single university hospital in Korea were analyzed. Among 50 MRSA strains, 13 strains had a deletion of mecI gene, and 37 strains were found to have mutations in mecI gene or mecA promoter region corresponding to a presumptive operator of mecA, i.e., the binding site of the repressor protein. Furthermore, in order to track the evolution of methicillin-resistant Staphylococcus aureus (MRSA) distributed in Korea, we determined the MRSA clonotype by combined use of genetic organization patterns of mec regulator genes, ribotype, and coagulase type. As the result, 48 of 50 MRSA strains could be classified into four distinct clones. Clonotype I is characterized by the coagulase type 3, deletion of mecI gene, and ribotype 1 shared by NCTC10442, the first reported MRSA isolate in England (9 strains). Clonotype II is characterized by the coagulase type 4, C to T substitution at position 202 of mecI gene, and ribotypes 2, 3 and 4 shared by 85/3619 strain isolated in Austria (10 strains). Clonotype III is characterized by the coagulase type 2, mutations of mecA promoter region and/or mecI, and ribotypes 4, 5, and 6 shared by N315 strain isolated in Japan (25 strains). Clonotype IV is characterized by the coagulase type 4, deletion of mecI gene, and ribotype 7 (4 strains). The clonality of two strains could not be determined due to their undefined ribotype.

Keywords

References

  1. Trend Microbiol v.2 Origin and evolution of DNA associated with resistance in Staphylococci Archer GL;Niemeyer DM
  2. Antimicrob Agents Chemother v.38 Dissemination among Staphylococci of DNA sequences associated with methicillin resistance Archer GL;Niemeter DM;Thanassi JA;Pucci MJ
  3. J Korean Med Sci v.3 In vitro activities of eight antibiotics against methicillin-resistant S. aureus and S. epidermidis strains isolated in Korea Chang WH;Choi MS;Chung HY;Seo WJ;Choi TY;Chong Y;Kim JS;Chung SS;Hong SH
  4. Microbial Drug Resist v.2 Ubiquitous presence of a homologue in natural isolates of Staphylococcus sciuri Couto I;Lencastre H;Severina E;Kloos W;Webster JA;Hubner RJ;(et al.)
  5. J Bacteriol v.158 Low-affinity penicillin-binding protein associated with β-lac-tam resistance in Staphylococcus aureus Hartman BJ;Tomasz A
  6. Microbiol Immunol v.39 Molecular evolution of MRSA Hiramatsu K
  7. Lancet v.350 Dissemmination in Japanese hospitals of strins of Staphylococcus aureus heterogeneously resistant to vancomycin Hiramatsu K;Arikada N;Hanaki H;Kawasaki S;Hosoda Y;Hori S
  8. FEBS Letters v.298 Molecular cloning and nucleotide sequence determination of the regulator region of mecA gene in methicillin resistant Staphylococcus aureus (MRSA) Hiramatsu K;Asada K;Suzuki E;Okonogi K;Yokota T
  9. J Infect Chemother v.2 Genetic basis for molecular epidemiologu of MRSA Hiramatsu K;Kondo N;Ito T
  10. Antimicrob Agents Chemother v.36 Survey of the methicillin resistance-associated genes mecA, mecR1-mecI, and femA-femB in clinical isolates of methicillin-resitant Staphylococcus aureus Hurlimann-Dalel RL;Ryffel C;Kayser FH;Berger-Bachi B
  11. J Clin Experi Med v.1 no.66 Coagulase typing on epidemiological study of methicillin-resistant Staphylococcus aureus Igarashi H
  12. J Biochem v.102 Nucleoetide sequence of the staphylocoagulase gene:its uniaue COOH-terminal 8 tanedm repeats Kaida S;Miyata T;Yoshizawa Y;Kawabata S;Morita H;Igarashi H(ed al.)
  13. Korean J Clin Microbiol v.2 no.S55 Vancomycin resistant Staphylococcus aureus in Korea Kim, MN;Hiramatsu K;Pai CH
  14. Antimicrob Agents Chemother v.42 Analysis of diversity of mutation sin the mecI gene and mecA promoter/operator region of methicillin-resistant Staphylococcus aureus and Staphylococcus epidermi8dis. Kobayahsi N;Koki T;Shozo U
  15. Antimicrob Agents Chemother v.40 Suppression of methicillin resistance in a mecA-containing pre-methicillin-resistant Staphylococcus aureus strain is caused by the mecI-mediated repression of PBP2 production Kuwahara-Arai K;Kondo N;Hori S;Tateda-Suzuki E;Hiramastsu K
  16. J Infect Chemother v.2 Characteristics of methicillin-resistant Staphylococcus aureus isolated from wounds in Korean patients Lee, MS;Chong Y
  17. J Clin Microbiol v.30 Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources:association of the mec gent with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination Musser JM;Kapur V
  18. Japanese J Infect Dis v.68 Antibiotic susceptibilities and other biological properties of methicillin-resistant Staphylococcus aureus: observation in the last 2 years in our university hospital Nakahara S;Kawayama T;Yokoyama T;Akiyoshi H;Okubo Y.(ed al.)
  19. National Committee fr Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically-fourth edition
  20. Diagnostic molecular microbiology Ribotyping in molecular epidemiology Popovic T;Bopp CA;Olsvik O;Kiehlbauch JA;Persing DH(ed);Smith TF(ed);Tenover FC(ed);White TJ(ed)
  21. Antimicrob Agents Chemother v.36 Correlation between regulation of mecA transcription and expression of methicillin resistance in Staphylococci Ryffel C;Kayser FH;Berger-Bachi B
  22. Korean J Infect Dis v.27 Antimicrobial resistance and plasmid profile of methicillin-resistant Staphylococcus aureus Ryu PY;Kim YI;Rhee JH;Chung SS;Ahn TH;Shin JH
  23. FEBS Letters v.221 Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion Song MD;Wachi M;Doi M;Ishino F;Matsuhashi M
  24. Antimicrob Agents Chemother v.37 Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains Suzuki E;Kuwahara-Arai KJ;Richardson F;Hiramatsu K
  25. J Infect Chemother v.1 A retrospective study on methicillin-resistant Staphylococcus study on methicillin-resistant Staphylococcus aureus clinical strains in Tokyo University Hospital Tanaka T;Okuzumi K;Iwamoto A;Hiramatsu K
  26. Antimicrob. Agents Chemother v.34 Evidence of a novel staphylococcal mec-encoded element (mecR) controlling expression of penicillin-binding protein 2 Tesch W;Ryffel C;Strssle A;Kayser FH;Berger-Bachi B;
  27. Antimicrob Agents Chemother v.28 Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphlococcus aureus Utsui Y;Yokota T
  28. Microbiol Drug Resist v.2 Tracking the evoutionary origin of the methicillin resistance gene: cloning and sequencing of a homologue of mecA from a methicillin susceptible strain of Staphylococcus sciuri Wu S;Piscitelli C;Lencastre H;Tomasz A