The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
권호 표지

Analysis of X Irradiation Related Genes in HL60 Cells Using cDNA Microarray

cDNA Microarray를 이용한 HL60 세포주에서 방사선 조사 관련 유전자의 검색 및 분석

  • Park, Keon-Uk (Department of Microbiology, Keimyung University School of Medicine) ;
  • Hwang, Mi-Sun (Department of Microbiology, Keimyung University School of Medicine) ;
  • Suh, Seong-Il (Department of Microbiology, Keimyung University School of Medicine) ;
  • Suh, Min-Ho (Department of Microbiology, Keimyung University School of Medicine) ;
  • Kwon, Taeg-Kyu (Department of Immunology, Keimyung University School of Medicine) ;
  • Park, Jong-Wook (Department of Immunology, Keimyung University School of Medicine) ;
  • Cho, Jae-We (Department of Microbiology, Seonam University College of Medicine) ;
  • Choi, Eun-Ju (Department of Obstetrics and Gynecology, Taegu Catholic University School of Medicine) ;
  • Baek, Won-Ki (Department of Microbiology, Keimyung University School of Medicine)
  • 박건욱 (계명대학교 의과대학 미생물학교실) ;
  • 황미선 (계명대학교 의과대학 미생물학교실) ;
  • 서성일 (계명대학교 의과대학 미생물학교실) ;
  • 서민호 (계명대학교 의과대학 미생물학교실) ;
  • 권택규 (계명대학교 의과대학 면역학교실) ;
  • 박종욱 (계명대학교 의과대학 면역학교실) ;
  • 조재위 (서남대학교 의과대학 미생물학교실) ;
  • 최은주 (대구가톨릭대학교 의과대학 산부인과학교실) ;
  • 백원기 (계명대학교 의과대학 미생물학교실)
  • Published : 2000.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Recently developed cDNA microarray or DNA chip technology allows expression monitoring of expression of hundreds and thousands of genes simultaneously and provides a format for identifying genes as well as changes in their activity. In order to search for changes in gene expression after X irradiation in HL60 cells, cDNA microarray technique was done. In this study, expression of 588 human genes (including oncogenes, tumor suppressor genes, cell cycle regulator genes, intracellular signal transduction modulator genes, apoptosis related genes, transcription factor genes, growth factors and receptor genes, cytokine genes, etc) were analyzed. For cDNA microarray analysis mRNAs were extracted from control and 8 Gy-irradiated HL60 cells. As a result the changes in expression of several genes were observed. This alteration of gene expression was confirmed by reverse transcription-polymerase chain reaction. The expression of heat shock 60 KD protein, c-jun, erythroid differentiation factor, CPP32, myeloid cell nuclear differentiation antigen, MAP kinase-activated protein kinase, interleukin-8, monocyte chemotactic peptide 1 and RANTES genes was increased, but the expression of p55CDC gene was decreased after X irradiation.

Keywords

References

  1. Nature v.392 Chemokines and leukocyte traffic Baggiolini M
  2. J Cell Biochem v.49 Cloning and expression of the human myeloid cell nuclear differentiation antigen: regulation by inter-feron alpha Briggs JA;Burrus GR;Stickney BD;Briggs RC
  3. Cell v.92 The Hsp70 and Hsp60 chaperone machines Bukau B;Horwich AL
  4. Br Med Bull v.51 Gene therapy for solid tumors Culver KW;Vickers TM;Lamsam JL;Walling HW;Seregina T
  5. Nat Genet v.14 Use of a cDNA microarray to analyse gene expression patterns in human cancer DeRisi J;Penland L;Brown PO;Bittner ML;Me-ltzer PS;Ray M;Chem Y;Su YA;Trent JM
  6. Trends Cell Biol v.8 The central executioners of apoptosis: caspases or mitochondria? Green D;Kroemer G
  7. Proc Natl Acad Sci USA v.88 Protein kinase C mediates x-ray inducibility of nuclear signal transducers EGR1 and JUN Hallahan DE;Sukhatme VP;Sherman ML;Viru-dachalam S;Kufe D;Weichselbaum RR
  8. Proc Natl Acad Sci Usa v.94 Discovery and analysis of inflammatory disease-related genes using cDNA microarrays Heller RA;Schena M;Chai A;Shalon D;Bedilion T;Gelmore J;Woolley DE;Davis RW
  9. Radi-at Red v.125 Mechanism of radiosensitization by halogenated pyrimidines: Effect of BradU on cell killing and interphase chro-mosome breakage in radiation sensitive cells Iliskis G;Pantelias G;Kurtzman S
  10. Exp Lung Res v.24 Alterations in the expression of chemokine mRNA levels in fibrosis-resistant and -sensitive mice after thoracic irradiation Johnston CJ;Wright TW;Rubin P;Finkelstein JN
  11. Cancer Res v.51 Participation of p53 protein in the cellular response to DNA damage Kastan MB;Onyekwere O;Sidransky D;Vogelstein B;Craig RW
  12. Cancer Res v.50 Possible role for p34cdc2 kinase in etoposide-induced cell death of Chinese hamster ovary cells Lock RB;Ross WE
  13. Nature Biotechnol v.14 Expression minitoring by hybridization to high-density oligo-nucleotide arrays Lockhart DJ;Dong H;Byrne MC;Follettie MT;Gallo MV;Chee MS;Mittmann M;Wang C;Kobayashi M;Horton H;Brown EL
  14. Mol Cell Biol v.16 kinase-activited protein kinase, is targeted by three MAP kinase pathways Ludwig S;Engel K;Hoffmeyer A;Sithanandam G;Neufeld B;Palm D;Gaestel M;Rapp UR
  15. Radiat Res v.62 Effects of ionizing radiation on DNA replication in cultured mammalian cells Makino F;Okada S
  16. Cancer Res v.53 Differen-tial effect of ionizing radiation on the expression of cyclin A and cyclin B in HeLa cells Muschel RJ;Zhang HB;McKenna WG
  17. Exp Hematol v.23 Effects of activin A/erythoid differenti-ation factor on erythroid and megakaryocytic diffe-rentiations of mouse erythroleukemia(Friend) cells: evidence for two distinct modes of cell response Okafuji K;Kaku K;Seguchi M;Tanaka H;Azuno Y;Kaneko T
  18. Cancer Res v.50 Antisense-mediated inhibi-tion of Bcl2 protooncogene expression and leukemic cell growth and survival: Comparision of phospho-diester and phosphorthioate oligodeoxynucleotides Reed JC;Stein C;Subasinghe C;Haldar S;Croce CM;Yum S;Cohen J
  19. Proc Natl Acad Sci USA v.93 Parallel human genome analysis: Mi-croarray-based expression monitoring of 1000 genes Schena M;Shalon D;Heller R;Chai A;Brown PO;Davis RW
  20. Biochem Biophys Res Commun v.167 Inducible gene expression of activin A/erythroid differentiation factor in HL-60 cells Takahashi S;Yamashita T;Eto Y;Shibai H;Mi-yamoto K;Ogata E
  21. Radiother Oncol v.47 The role of the stress-activited protein kinase (SAPK/JNK)signaling pathway in radiation-induced apop-tosis Verheij M;Ruiter GA;Zerp SF;van Blitterswijk WJ;Fuks Z;Haimovitz-Friedman A;Bartelink H
  22. Trends Biochem Sci v.22 Caspases and caspase inhibitors Villa P;Kaufmann SH;Earnshaw WC
  23. Mol Med Today v.3 DNA on a chip: serving up the geno-me for diagnostics and research Wallace RW
  24. Proc Natl Acad Sci USA v.95 Mad2 transiently associ-ates with an APC/p55Cdc complex during mitosis Wassmann K;Benezra R
  25. Mol Cell Biol v.14 A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharo-myces cerevisiae cell division cycle proteins Cdc20 and Cdc4 Weinsten J;Jacobsen FW;Hsu-Chen J;Wu T;Baum LG
  26. EMBO J v.18 Hsp60 accelerates the maturation of pro-caspase-3 by upstream activator proteases during apoptosis Xanthoudakis S;Roy S;Rasper D;Hennessey T;Aubin Y;Cassady R;Tawa P;Ruel R;Rosen A;Nicholson DW
  27. Exp Hematol v.25 MNDA binds NPM/B23 and the NPM-MLF1 chimera generated by the t(3;5) as-sociated with myelodysplastic syndrome and acute myeloid leukemia Xie J;Briggs JA;Morris SW;Olson MO;Kinney MC;Briggs RC